中国静灰球体外抑菌活性的研究.pdfVIP

第6卷第2期 菌 物 研 究 V01．6 No．2 2008年6月 Journal of Fungal Research June 2008 中国静灰球体外抑菌活性的研究 林 养 ，齐 晋 ，张火财2，张凌凌 ，王 琦 (1．吉林农业大学中药材学院，长春130118；2．北京大北农动物保健科技有限责任公司，北京 100085) 摘 要：采用纸片扩散法(Kirby—Bauer法)和菌饼法(活菌活性测定法)，系统研究了中国静灰球的菌丝体、发 酵液、活菌菌饼及其子实体的水、乙醇、乙酸乙酯、乙醚、三氯甲烷和石油醚6种溶剂提取液对金黄色葡萄球 菌、大肠埃希氏菌、鼠伤寒沙门氏菌、黑曲霉和绿色木霉5种测试菌的体外抑菌活性。结果表明：中国静灰球 的菌丝体、发酵液及活菌菌饼对金黄色葡萄球菌、鼠伤寒沙门氏菌和黑曲霉均有不同程度的抑菌作用，即具有 广谱的抑菌活性；其水和乙酸乙酯的浸提液对金黄色葡萄球菌均具有较显著的抑菌作用。推测中国静灰球菌 丝体中含有对金黄色葡萄球菌、大肠埃希氏菌、鼠伤寒沙门氏菌、黑曲霉、绿色木霉5种测试菌的广谱抑菌活 性物质。 关键词：中国静灰球；马勃；抑菌活性；金黄色葡萄球菌；大肠埃希氏菌；鼠伤寒沙门氏菌；黑曲霉；绿色木 霉 中图分类号：R285．5 文献标识码：A 文章编号：1672—3538(2008)02—0110—04 Study on the Antimicrobial Activities of Bovistella sinensis in Vitro LIN Yang’ Qf Jin’，ZHANG Huo—cai ，ZHANG Ling—ling’ WANG Q ’ ， ， (1．College ofChinese Medicinal Materials，倒in Agricultural University，Ch．angchun 130118，China； 2．Peking Dabeinong Animal Health Care Science and Technology Co．Ltd．，Beijing 100085，China) Abstract：The antimierobial activities of the mycelia and the broth of Bovistella sinensis Lloyd and solvent extractions of water，ethanol，ethyl acetate，ethyl ether，trichloromethane and petroleum ether from the fruiting bodies on Staphylococcus aureus，Escherichia coli，Salmonella typhimurium，Aspergillus niger and Trichoderma viride in vitro were systematically studied，using the method of Kirby—Bauer(KB)and fungus cake．The mycelia，山e broth and the solvent extractions were of different antimicrobial activities to S．aureus，S．typhimurium and A．niger，SO they had the quality of broad spectrum；the solvent ex— tractions of water and ethyl acetate were of the comparatively significant antimicr