镉染毒对大鼠骨生物力学性能的影响 - 中国实验动物学报.doc

周传农修改： 英文摘要 — 已修改 正文中的英文 — 已顺 图与表的英文 — 已顺 文献的英文书写 — 已顺 镉染毒对雄性大鼠骨生物力学性能的影响 秦波音1，陈晓2 ，朱国英2, 周晓辉1，周文江1,3( （1. 上海市公共卫生临床中心，上海 201508；2. 复旦大学放射医学研究所，上海 200032；3.复旦大学药学院动物中心，上海 201203） 【摘要】 目的 探讨镉对大鼠股骨和腰椎生物力学性能的影响。方法 24只8周龄雄性SD大鼠随机分成4组，分别背部皮下注射生理盐水0.5 mL （对照组）和0.1 mg/kg bw (低剂量组)，0.5 mg/kg bw（中剂量组），1.5 mg/kg bw（高剂量组）CdCl2，每周称体重，并根据体重调整注射量。染毒后第12周，收集全血、腰椎及股骨，分别用于血镉测定、骨镉测定、骨密度测定及生物力学测定。结果 染毒组大鼠体内血镉及骨镉水平明显高于对照组，差异有统计学意义（P 0.05）；中、高剂量染毒组大鼠骨密度较对照组显著下降（P 0.05）；染毒组大鼠股骨和腰椎生物力学性能较对照组有不同程度的的降低，其中高剂量染毒组大鼠股骨生物力学性能的下降和对照组相比有显著差异（P 0.01）；中剂量和高剂量染毒组大鼠腰椎生物力学性能和对照组相比差异有统计学意义（P 0.01）。结论 镉影响大鼠股骨和腰椎的生物力学性能，并且腰椎较股骨更为敏感。 【关键词】 镉；大鼠；骨密度；生物力学 Effects of cadmium on bone biomechanical properties in male rats QIN Bo-yin1, CHEN Xiao2, ZHU Guo-ying2, ZHOU Wen-jiang1,3(1. Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China; 2. Institute of Radiation Medicine, Fudan University, Shanghai 200032；Abstract】 Objective To investigate the effects of cadmium (Cd) on biomechanical properties of femur and lumbar spine in male rats. Methods Twenty-four 8-week-old Sprague-Dawley male rats were randomly divided into 4 groups which were given CdCl2 by subcutaneous injection at a doses of 0 (sodium chloride), 0.1, 0.5 and 1.5 mg /kg body weight, respectively, 6 rats in each group. At the 12th week, blood, lumbar spine and femur were collected for cadmium assay, bone mineral density (BMD) measurement and biomechanical test. Results The Cd content in blood and bone of Cd-treated rats were significantly higher than that in the control group (P 0.05). BMD of rats exposed to 0.5 mg/kg·bw and 1.5 mg/kg·bw Cd were significantly lower than that of the control group (P 0.05). Biomechanical properties of the femur and lumbar spine in the Cd-treated rats were decreased compared with the control, especially for the biomechanical properties of femur in the 1.5 mg/kg·bw Cd group and the biomechanical properties of lumbar spine in the 0.5 and 1.5 mg/kg·bw Cd groups