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Preparationofacuteretinalnecrosissyndromemodelinrabbits
MengXiang—jun’,SuYun’,WuCa—zhen,LiHong’,SuJing’
Abstract
Departmentof
BACKGROUND:Acuteretinalnecrosissyn~ome(ARNS)isaseriousoculardiseasewhichcanleadtoblindnessAtpresent.
Ophthalmology,
A墒liatedZhongshan herpessimplexvirusorvraicella—hepreszosterviursisconsideredasamainalgogen.Toestablishanem cientandreasonableanimal
Hospital,Dalian mode1isanecessaryprocedureinARNSstudies.
University,Dalian
116o01.Liaoning OBJECTIVE:ToestablishARNSmodelinrabbitsbyherpessimplexvirustype1(HSV一1)wasinjectedintotherabbiteyes.
Province,China; DESIGN,TIM EAND SETTING:Theexperiment,randomizedcontroltria1.wasfulfilledinthecentrallaboratoryofhteFirst
Departmentof HospitalofJilinUniversity,AnimalExperimentRoom oftheSecondHospitalofJilinUniversity,andBasicMedicalCollegeofJilin
Ophthalmology,
Universityfrom FebruarytoOctoberin2006.
SecondHospitalof
JilinUniversity, MATERIALS:Fortyhealthyadultgreyrabbitsofeihtergender,weighing(2.5±o.5)kgwereusedinthisstudy.HSV一1SM44strain
Changchun130O41. waspurchasedfrom theThirdDepartmentofVaccineiuNationa1InstitutefortheControlofPharmaceuticalandBiologicalProducts.
JilinProvince,China
VerocellswereofferedbyChangchunInstituteofBiologicalProducts.
MengXiang-jun☆. METHoDS:HSV一1wascultured.na dhteARNSmodelingreyrabbitswasdividedintotwogroups.Experimentalgroupreceived
Doctor,Physician,
iniectionof1O uLasepticHSC一1SM44viurssolutionintohteretinaofrighteye.Contro1groupwasiqectedwithmesalnevolume
Departmentof
Ophthalmology, ofVerocellsupematants.
AfflliatedZhongshan MAIN 0UTC0MEMEASURES:Theofndusphotographywasobservedat2,4,7,10,12,14,2l,28daysna d6weeks
Hospita1.Da1ian
口Ostoperativelv.Thena imalswerekiliedandtheeyeswerehravested.Thehistologicalobservationnadtransmissionelectron
University,DalJan
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