子宫内膜异位症在位内膜多效营养因子和中期因子的表达研究.pdfVIP

一 478一 重庆医科大学学报2008年第33卷第4期 (Journal of Chongqing Medical University 2008．VO1．33 No．4) 謦 文章编号：0253—3626(2008)04—0478—03 子宫内膜异位症在位内膜多效营养因子和中期因子的表达研究 杨 蕾，赵纯全，令狐华 (重庆医科大学附属第一医院妇产科，重庆 400016) 【摘 要】目的：探讨多效营养因子(Pleiotrophin，PTN)和中期因子(Midkine，MK)蛋白在子宫内膜异位症(Endometriosis，EMs)患 者在位内膜组织中的表达变化及临床意义。方法：应用免疫组化链霉菌抗生物素蛋白一过氧化物酶连接(sP)法，检测30例 EM 患者(研究组)的在位内膜组织及29例子宫肌瘤患者(对照组)的内膜组织中FrrN和MK蛋白的表达。结果：PTN蛋白主要 在子宫内膜腺上皮细胞浆和细胞膜中表达，而MK蛋白阳性表达也主要定位于子宫内膜腺上皮细胞的细胞浆，部分标本细胞核 也有阳性表达。EMs组中FrrN和MK蛋白表达均较对照组显著升高(P均0．001 o结论：FrrN和MK蛋白在EMs患者的在位内 膜过度表达，导致其血管生成能力增强。EMs在位内膜中PrN和MK蛋白的表达变化可能与EMs的发生发展有关。 【关键词】多效营养因子；中期因子；子宫内膜异位症；新生血管 【中国图书分类法分类号】R71 【文献标识码】A 【收稿日期】2007—08—20 Expression study of pleiotrophin and midkine in endometrium of endometriosis ⅣG ，Je ，e (Department of Obstetrics and Gynecology，the First Affiliated Hospital，Chongqing Medical University) 【Abstract】Objective：To study the expression of Pleiotrophin and Midkine protein in endometrium of endometriosis and its clinical significance．Methods：Expressions of Pleiotrophin and Midkine protein were examined by immunohistochemical staining method in 30 endometrial samples of endometriosis(study group)and 29 endometrium of uterine fibroid(control group)．Result： Pleiotrophin protein mainly expressed in the membrane and plasma of glandular epithelium of endometrium．Midkine protein mainly expressed in the plasma of grandular epithelium of endometrium and expressed in nuclei in some samples．The expression of Pleiotrophin and Midkine protein in endometriosis was significantly higher than that in the control group(PO．001)．Conc~一 s o7 ：over-expression of FrrN and MK protein in endometrium of endometriosis correlated to endometriotic cell angiogenesis．The change of FrrN and MK expression in endometrium of endometriosis may relate to the patho