玉米泛素延伸蛋白基因zmerd16 的克隆、序列特征和表达 - 作物学报.pdf

作物学报 ACTA AGRONOMICA SINICA 2010, 36(7): 1075−1083 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2010.01075 玉米泛素延伸蛋白基因ZmERD16 的克隆、序列特征和表达分析 陆月赏1,2 刘颖慧2,3,** 张登峰2 石云素2 宋燕春2 王天宇2,* 杨德光1,* 黎 裕2 1 , 150030; 2 , 100081; 3 , 075000 : EST ERD16 AtERD16 , , ZmERD16 ZmERD16 390 bp, 129 , pI 9.94, 14.7582 kDZmERD16 1 , 53 , ZmERD16 4 3 , ZmERD16 , PCR ZmERD16 , ZmERD16 , PEG ZmERD16 : ; ; ; ; ZmERD16 Identification and Expression Analysis of ZmERD16 : a Ubiquitin Extension Protein Gene in Maize (Zea mays L.) 1,2 2,3,** 2 2 1 LU Yue-Shang , LIU Ying-Hui , ZHANG Deng-Feng , SHI Yun-Su , SONG Yan-Chun , WANG Tian-Yu2,* , YANG De-Guang1,*, and LI Yu2 1 College of Agronomy, Northeast Agricultural University, Harbin 150030, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sci- ences, Beijing 100081, China; 3 Hebei North University, Zhangjiakou 075000, China Abstract: Ubiquitin extension protein is a fusion protein of an ubiquitin monomer followed by a ribosomal protein with 52–53 animo acids or 76–78 amino acids. Ubiquitin extension proteins have been paid attention that they play important roles in re- sponding to stresses and regulating certain developmental processes in plants. We describe here the isolation, sequence character- istics and expression analysis of ZmERD16 gene encoding a homologue of ubiquitin extension protei