猪卵母细胞蛋白质组双向电泳体系的建立及初步分析 - 动物学杂志.pdf

猪卵母细胞蛋白质组双向电泳体系的建立及初步分析 - 动物学杂志.pdf

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猪卵母细胞蛋白质组双向电泳体系的建立及初步分析 - 动物学杂志

Chinese Journal of Zoology 2008,43(4) :67~ 71 * 胡惠忠 付 强 卢晟盛 张 明 陆阳清 蒙 冰 卢克焕 ( 530004) : ( Sus scrof a) , , , ImageMaser , 800 , 35 ,22 13 : ; ; ; : Q492 :A : 2008) 046705 Twodimensional Electrophoresis Analysis of Porcine Oocyte Proteome: a Preliminary Study HU Hui hong FU Qiang LU ShengSheng HANG Ming LU YangQing * MENG Bing LU KeHuan ( GuangxiKey Laboratory of Subtropical BioResource Conservation and Utilization, Guangxi University , anning 530004, China) Abstract:The twodimensional electrophoresis ( 2DE) technique for Porcine ( Sus scrof a) oocyte proteome analysis was established. The components of protein lysis solution, sample treatment, and 2DE procedure were optimized. A profile of proteins extractedfrom Porcine oocyteswas obtained clearly. The protein expression profiles of immature and invitro matured Porcine oocytes were compared. The ImageMaster 2DE software was used to characterize different proteins. About 800 protein spots were identified, of which 22 proteinswere upregulated and 13 proteins were down regulated during oocyte maturation in vitro. It proves that proteomics technique base on twodimensional electrophoresis can be used in further studies on differential protein expressions in Porcine oocyte development. Key words: Porcine; Oocyte; Twodimensional electrophoresis; Proteomics (proteome) [ 1] , (twodimensional electrophoresis, 2 , DE) ( proteomics) [ 2,3] ( No. , (No. 0640002,0447004) ; (isoelectric poin

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