中华卵索线虫雌雄成虫可溶性蛋白双向电泳分析3 - current .pdf

动物学报 　50 ( 1) : 141 - 144 , 2004 A cta Zoologica S inica 　　　　　 　 中华卵索线虫雌雄成虫可溶性蛋白双向电泳分析 高　原　王国秀 　陈思礼 华中师范大学生命科学学院 , 武汉 430079 Preliminary analysis on dissoluble protein of Ovomermis sinensis adults by 2D PAGE GAO Yuan , WAN G GuoXiu , CHEN SiLi College of Life Sciences , Central China Normal University , Wuhan 　430079 , China Abstract 　Ovomermis sinensis (Nematode , Mermithidae) is a parasitic nematode of armyworm. But during its culture in vit ro and rearing in vivo , we found that we could not attain the suitable proportion of male and female nematodes. In order to disclose the reason why the number of the filial generation was imbalanced between two sexes and get basic data on genetic development , we analyzed dissoluble proteins of Ovomermis sinensis imagoes with electrophoresis. Firstly , through SDSPA GE , we found more than three specific proteins were detected in female imago with the approximate molecular weight of 15 - 16 kD , 21 - 24 kD , 72 - 76 kD and one about 26 kD specific protein in male imago. Secondly , we used a new improved twodimensional electrophoresis method to analyze the proteins and found that the result accords with those of SDSPA GE , which showed that this 2DE method is practicable. Also , in this 2DE , proteins can be well separated and plenty of different proteins can be clearly displayed [ Acta Zoologica Sinica 50 ( 1) : 141 - 144 , 2004 ]. Key words 　Ovomermis sinensis , 2DE , dissoluble proteins , different proteins 关键词 　中华卵索线虫 　双向电泳 　可溶性蛋白 　差异蛋白质 　　昆虫寄生线虫 ( 又称虫生线虫、昆虫病原线 用 2DE 技术 , 比较了中华卵索线虫雌雄成虫可溶 ) 虫 是本世纪发展起来的一种有潜能的生物防治因