玉米蛋白制备降血压肽的研究.pdf

290 2007, Vol. 28, No. 12 食品科学 ※生物工程 黄家音，朱宇洁，沈金玉 * (清华大学化学工程系生化研究所，北京 100084) ：本实验以我国来源丰富且价格低廉的玉米蛋白为原料，选用嗜热菌蛋白酶、胰凝乳蛋白酶、胰蛋白酶 和碱性蛋白酶等4种酶来水解玉米蛋白制备降血压肽。选用马尿酰-组氨酰-亮氨酸(HHL)为血管紧张素转化酶(ACE) 的模拟底物，利用反相高效液相色谱(RHPLC)检测ACE催化反应生成的马尿酸(Hip)的量，确定水解产物的ACE抑 制活性从而来对酶进行筛选。研究结果表明，碱性蛋白酶的水解能力最强，碱性蛋白酶和嗜热菌蛋白酶的水解产 物都达到了很好的ACE 抑制活性 (90% 左右)。 ：玉米蛋白；血管紧张素转化酶；降血压肽 Study on Producing Antihypertensive Peptides from Zein HUANG Jia-yin，ZHU Yu-jie，SHEN Jin-yu* (Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China) Abstract ：In this study, zein, a very abundant and cheap crop in China was hydrolyzed by thermolysin, chymotrypsin, trypsin and alkaline protease for processing antihypertensive peptides. Hippuryl-Histidyl-Leucine (HHL) was used as the simulant substrate of Angiotensin Converting Enzyme (ACE), and reverse-phase performance liquid chromatography (RHPLC) was used to measure the yield of Hippuryl (Hip). According to this process, the ACE inhibitory activity of each hydrolyzate was determined and chosen as the best protease. The results showed that alkaline protease has the optimum hydrolytic capability, while the hydrolyzate of alkaline and thermolysin shows great ACE inhibitory activity (up to 90%). Key words：zein；angiotensin converting enzyme(ACE)；antihypertensive peptides 中图分类号：Q51 文献标识码：A 文章编号：1002-6630(2007)12-0290-04 高血压是一种以动脉收缩压或舒张压升高为特征的 白的降血压肽对高血压患者可起到降压作用，对血压正 临床综合症，近年来，高血压在世界范围内成日趋严