牛红细胞源抗菌肽的分离纯化与活性鉴定.pdf

38 2 () Vol. 38 No. 2 2010 2 Journal of Northwest AF University( Nat. Sci. d.) Feb. 2010 * 胡建和, 杭柏林, 王 青, 董永军, 王三虎, 刘兴友 ( , 453003) [ ] (Antimicrobial peptides,AMPs) , ,( RPHPLC) ,, ; RPHPLC , 4 ( F1F2F3 F4) , ,F1 F3 , F1 ; ,F 1 2 562.40 u AMPs; F1 AM Ps , [ ] ; ; + [ ] S859.79 7 [ ] A [ ] 2010) Isolation, purification and act ivity ident ificat ion of ant imicrobial pept ides from bovine red blood cells HU Jianhe, HANG Bolin, WANG Qing, DONG Yongjun, WANG Sanhu, LIU Xingyou (S chool of A nimal Sc ience, H enan Institute of Sc ience and Technolog y , X inx iang, H enan 453003, China) Abstract : ObjectiveAntimicrobial peptides were isolated and purified from bovine red blood cell, and their antimicrobial activities in vitro were analyzed. Method Antimicrobial peptides from bovine red blood cells were isolated by ion exchange chromatography and purified by RPHPLC.Antimicrobial activity of purified peptide was tested by agrose radial diffusion assay. Molecular mass of purified peptidewhich had good antimicrobial activity was determined by mass spectrography.Result T he cation peak acquired by ion exchange chromatography had antimicrobial activity.Four peaks acquired by RPHPLC had the activity against E. coli, peak F1 and peak F3 had the activity against S ta hy lococcus aureus, only peak F1 had the activity against Candida albicans. Molecular mass of purified peptide of peak F1 was 2 562. 40 u.Conclu sionAntimicrobial peptides were isolated and purified successfully from bovine red blood