镍离子诱导牛血清白蛋白构象变化的动力学机理研究 - 河北科技大学学报.pdfVIP

29 4 Vol. 29, No .4 2008 12 Journal of Hebei University of Science and T ec nology Dec. 2008 : 1008-1542( 2008) 04-0283- 03 1 1 2 1 1 王改珍 , 苗凤智 , 刘英华 , 孟凡涛 , 张晓晓 ( 1. 河北科技大学环境科学与工程学院, 河北石家庄 050018; 2. 河北科技大学分析测试研究中心, 河北石家庄 050018) : 用差示紫外吸收光 法研究了镍离子与牛血清白蛋白( BSA) 相互作用的动力学过程, 发现 它们之间的相互作用是一个连串反应过程, 首先是镍离子与BSA 的 一个快速络合反应, 然后是镍 离子诱导BSA 发生了构象变化, 这 个构象变化过程是一个正向和逆向均为 一级反应的对峙反应, 而不是文献报道的简单一级反应 : 牛血清白蛋白; 镍离子; 相互作用; 滞后效应 : O657. 3 : A M ec anism for conformation transition of BSA induced by Ni ion 1 1 2 1 1 WANG Ga-i z en , MIAO Feng-z i , LIU Ying- ua , MENG Fan-tao , ZHANG Xiao-xiao ( 1. College of Environmental Science and Engineering, Hebei University of Science and Tec nology , S ijiaz uang H ebei 050018, C ina; 2. H ebei Researc Centre of Analysis and Testing, H ebei University of Science and Tec nology, S ijiaz uang H ebei 050018, C ina) Abstract: T e kinetics of conformational transition in t e interaction of Ni ion w it bovine serum albumin ( BSA) was studied using UV-Visible spectrometry and optical rotation measurement. T e results s ow ed t at t e interaction betw een BSA and Ni ion comprised of tw o consecutive reactions. T e first reaction was a fast coordination reaction between Ni ion and BSA; t e second reaction w as t e conformational transit ion of BSA induced by Ni ion. T e process w as not a simple first order reaction report ed by previous papers but an opposite react ion, w ic comprised of a forw ard f irst order reaction and reversible first order reaction. Key words: bovi