反相离子对色谱法测定缓释凝胶中二氧化氯含量 - 中国药事.pdfVIP

2010 24 3 235 论 著 胡超云, 谢 茵, 全 红, 毕小平 ( , 030001) : 建立反相离子对色谱法测定缓释凝胶中二氧化氯的含量 色谱柱为Diamonsil C18柱, 1 1 检测波长为260nm, 动相为385mmol L 氢氧化四 丁基铵的水甲醇 ( 20 80) , 速为 1mL min , 1 柱温为25 ! , 进样量为20L 二氧化氯在 187~ 23881mg L 范围内与峰面积呈良好的线性关系 ( r = 09997, n = 8) , 平均回收率为9814% , RSD 为186% ; 日内日间精密度的RSD 分别为022% ~ 123%036% ~ 137% 该方法快速, 准确, 简便, 可用于凝胶中二氧化氯的含量测定 : 二氧化氯; HPLC; 离子对 : R917; R9272 : A : ( 2010) Determination of Chlorine Dioxide in Delayed Released Gel by Ionpair RPHPLC Hu Chaoyun, Xie Yin, Quan H ong and Bi Xiaoping ( College of Pharmaceutics, Shanxi M edical U niversity, T aiyuan 030001) ABSTRACT: Ob ective To establish ionpair RPHPLC to determine the contents of chlorine dioxide in Delayed Released Gel. Methods Diamonsil C18 w as the chromatographic column; 260nm w as the UV 1 detection w avelength; the mobile phase w as w ater: methanol ( 20 80) , included 385mmol L T BAOH. 1 T he flow rate w as 1mL min ; the column temperature w as 25 ! ; the sample size w as 20L. Results T he concentration of chlorine dioxide had a good linear relationship w ith the peak area in the range of 187~ 1 23881mg L . The average recovery was 9814% ; RSD w as 186%; the precision of w ithin day and daytime w as 022%~ 123% and 036%~ 137%, respectively. Conclusion T he method is quick, accurate and convenient for determining the contents of chlorine dioxide. KEY WORDS: chlorine dioxide; HPLC; ionpair [ 12] [ 710] ( Stabilized Chlorine Dioxide) , , ,