益肾饮对雷公藤多甙治疗大鼠系膜增生性肾小球肾炎抗生育活性影响的探究.pdfVIP

提 要 目的：探讨益肾饮对雷公藤多甙治疗大鼠系膜增生性肾小球肾炎抗生育活性的影 响。方法：50 例 Wistar 大鼠（50 天龄）随机分为正常对照组、模型组、雷公藤多 甙组、益肾饮组、合用组,后四组制备系膜增生性肾小球肾炎模型。药物干预三组于 造模第 5 周起,分别开始灌服雷公藤多甙、益肾饮、雷公藤多甙与益肾饮合用进行干 预治疗,模型组和正常对照组每天给予自来水灌胃。分别于实验前、12周末分别留取 并测定24小时尿蛋白定量。12周末全部处死大鼠后抽取血化验睾酮（T）、孕酮（P）、 雌二醇（E ）等各种指标。对比睾丸、卵巢、子宫、肾的病理结构及睾丸、卵巢中一 2 氧化氮合酶（NOS）表达的变化。结果：与模型组比较,益肾饮能减轻系膜增生性肾炎 的各种表现,益肾饮与雷公藤多甙合用后疗效更明显；与雷公藤多甙组比较,益肾饮与 雷公藤多甙合用后能拮抗雷公藤多甙对生殖系统的损害,上调睾丸、卵巢中NOS表达。 结论：益肾饮可能通过影响生殖器官NOS活性来对抗雷公藤多甙治疗系膜增生性肾小 球肾炎时对生殖系统的损害。 关键词 雷公藤多甙；系膜增生性肾小球肾炎；益肾饮；抗生育活性 The Effects of Yishenyin on Antifertility Activity of Rats with MsPGN Treated by Glucosidorum Tripterygll Totorum Speciality：Internal medicine of TCM Author ：Wang Yaoyao Tutor：Professor Yu Junsheng Abstract Objective: To observe the effects of Yishenyin on antifertility activity of rats with MsPGN treated by Glucosidorum Tripterygll Totorum. Methods: 50 normal Wistar rats (50days’s age) were randomly divided into normal group, model group, Glucosidorum Tripterygll Totorum group, Yishenyin group and common group. The rats in last four groups were established rat models of mesangial proliferative glomerulonephritis respectively. From 5 weeks, Glucosidorum Tripterygll Totorum, Yishenyin and combined Glucosidorum Tripterygll Totorum and Yishenyin were given to the rats in the last three groups respectively. While rats in normal group and model group were given normal water of same quantity as a control. 24 hour urine protein excretion was observed before the experiment and 12 weeks. Varies of indexes were observed in 12 weeks when all the rats were executed, including testosterone (T), progesterone (P), estradiol (E2). Kidney, testicle, ovary and uterus pathological