补肾中药生精方对肾阳虚男性不育患者精子DNA印记基因H19表达的探究.pdfVIP

提 要 目的：观察补肾中药生精方对肾阳虚患者精子DNA甲基化，印迹基因H19的表达状态的 影响，探讨补肾中药改善少弱精子症患者精子质量的作用机理。 方法：临床研究将67例因少弱精子症而不育的患者随机分为两组，分别用生精方 （连 方教授自拟方）治疗3个月（治疗组，34例）和五子衍宗丸方处治疗3个月后（对 照组，33 例），提取患者精液进行PCR 、RT-PCR 结合 RFLP 检测印迹基因H19在少 弱精子症中的印迹状态，然后检测两组患者肾阳虚证候评分变化、治疗前后精液常规 检查的变化，包括精子数量、活力、液化状态、精子畸形率等，结合临床妊娠率进行 评定。 结果：（1）两组患者肾阳虚症状均有所改善，但治疗组比对照组疗效显著，前后证候 评分比较有统计学意义（P0.05）；（2）治疗组精子数量、活力明显优于对照组 （P0.05），正常形态率的改变前后无统计学意义；（3）治疗组临床妊娠率明显优于 对照组，差异有统计学意义(P0.05)；（4）治疗组印迹基因H19有表达，但治疗组表 达有改善的趋势，但无统计学意义，两者比较差异有显著性（P0.05）） 结论：补肾中药生精方通过改善不育患者的精液质量，进而提高临床妊娠率，其疗效 机理可能与改善精子DNA印迹基因H19的表达有关。 关键词 补肾；精液数量及动力等级；H19；生精方 Born in patients with kidney Yang pure party sperm DNA methylation influence CTmRNA Expression of Research Speciality:Gynecology of TCM Author :guo liang Tutor :Professor Lian Fang Abstract Objective: Watch birth of traditional Chinese medicine pure party nourishing kidney patients with Yang sperm DNA methylation, H19 gene expression state mark, the effect of traditional Chinese medicine to improve less discussed kidney disease patients weak sperm function mechanism of sperm quality. Methods: clinical research 67 patients with less because of weak sperm disease of infertility patients were randomly divided into two groups, with pure party respectively born (even the party to professor from party) deal with 3 months (the treatment group, 34 cases) and traditional Chinese medicine from the Pope pill abital disposal after 3 months (control group, 33 cases were treated and extraction patients sperm positive PCR, the RT-PCR detection H19 gene combination RFLP molecularly imprinted in weak sperm molecularly imprinted in the less disease state; And then testing two groups were symptoms of kidney Yang integral change, before a