解毒活血中药伍对载脂蛋白E基因敲除小鼠瘀毒模型的干预作用.pdfVIP

提 要 目的：以血脂康为对照，观察不同剂量解毒活血中药大黄加红曲配伍对载脂蛋白 E （Apolipoprotein E ，ApoE ）基因敲除小鼠瘀毒模型血脂、主动脉粥样硬化病理形态 学、全血粘度、炎症因子及细胞凋亡的影响。方法：6 周龄 ApoE 基因敲除 [ApoE(-/-)] 小鼠，随机分为瘀毒模型组、大黄加红曲大剂量组、大黄加红曲中剂量组、大黄加红 曲小剂量组、血脂康组、ApoE （-/- ）小鼠模型对照组，同时选取相同遗传背景的同 龄正常 C57BL/6J 小鼠作为正常对照组。前 5 组每周 2 次注射内毒素造模，各给药组 连续用药 8 周后，取血检测各组小鼠血脂（TC、TG、HDL-C 及 LDL-C ）水平、全血 粘度、炎症因子（TNF- α、IL-1、IL-6 ）的浓度；取主动脉、心脏进行病理形态学观 察，免疫组织化学法检测 Bcl-2 、Bax 蛋白表达。结果：1. 解毒活血中药大黄加红曲 配伍能降低瘀毒模型小鼠的全血粘度、血清 TC、TG、LDL-C 、TNF- α、IL-1、IL-6， 升高血清 HDL-C 水平，保护动脉及心肌形态结构，使主动脉和心肌的 bcl-2 表达增强， bax 表达减弱，对 ApoE(-/-)小鼠瘀毒模型动脉粥样硬化的斑块形成有对抗作用。结论： 本研究表明解毒活血中药大黄加红曲配伍具有降脂、抗炎、降低血粘度、抑制凋亡、 保护动脉及心肌结构等作用，对防治动脉粥样硬化和预防急性冠脉综合征等心脑血管 事件有重要意义。 关键词 解毒活血中药配伍；载脂蛋白 E 基因敲除小鼠；内毒素；瘀毒模型；动 脉粥样硬化 The Mechanical Study on the intervention of combination with toxin-resolving and blood-activating herbs in the Atherosclerosis of stagnation-poison model from apolipoprotein E gene knockout mice Speciality: Basic Research of Integrated TCM and Western Medicine Author: Zhang Jianying Tutor: Prof. Zheng Guangjuan Abstract Objective:Compared with Xuezhikang,to observe the effect of different doses of pieplant and red rice that were combination with toxin-resolving and blood-activating herbs on blood lipids, aorta atherosclerosis,blood viscosity,inflammatory factors and apoptosis of stagnation-poison model from apolipoprotein E gene knockout mice.Methods:Six –week old ApoE(-/-) mice were divided randomly into six groups:stagnation-poison model group,high-dosage-treatment group(combination of pieplant and red rice),middle- dosage-treatment-group(combination of pieplant and red rice),low-dosage-treatment- group (combination of pieplant and red rice), model-comparison group, Xuezhikang group. Six-week-old heathy mice (C57BL/6J mice) were the normal