双水相体系萃取分离葡萄籽多糖 - 中国酿造.pdf

中 国 酿造 2013年第32卷第7期 研究报告 总第256期 ·89· 双水相体系萃取分离葡萄籽多糖 孙亚莉，张瑾，裴淑平，刘洋，李鑫文，张喜峰木 (河西学院农业与生物技术学院，甘肃张掖734000) 最陡爬坡试验逼近最大萃取率区域，然后通过响应面组合试验对显著因素进行优化。得到最佳萃取条件为：PEG2000质量分数为 8．92％、(NH4)2So。质量分数为16．41％、萃取温度为38℃，萃取率可达55．1％，与预测值56．075％接近。 关键词：双水相萃取；最陡爬坡试验；多糖 中图分类号：TS209 文献标识码：A 文章编号：0254—5071(2013)07—0089—05 Extractionand from seeds separationofpolysaccharidegrape bytwo-phaseaqueoussystem SUN Yali，ZHANGJin，PEIShuping，LIUYang，LIXinwen，ZHANGXifeng+ and (TheCollegeofAgricultureBioteclmology(CAB),HexiUniversity,Zhangye73400谚China) Abstract：Extractionand from seeds was Plackett bytwo-phaseaqueoussystemsinvestigated separationofpolysaccharidegrape by ascent and surface was to themassfractionofPEG2000 sign，steepestdesignresponsemethodology．Firstly，PBdesignappliedidentify andextraction asthemost of5factors theextraction．Thenthecenter ofthethreefactorsweredetermined temperature significant influencing points basedon ascent the ofthethreefactorsWascarriedout surface results by response methodology．The steepestdesign．Thenoptimization using showedthatthebestextractconditionswereasfollows：themassfractionsofPEG2000massfractions 8．92％，the of(NH4)2S04 at38。C．Under extractionratewas oneof56．075％． suchconditions，the 55．1％，whichwasingood agreementwiththepredicted