正交试验优化超声法提取北苍术多糖的工艺研究 - 天津中草药杂志社.pdfVIP

现代药物与临床 Drugs Clinic 第 27 卷 第 5 期 2012 年 9 月 • 457 • 正交试验优化超声法提取北苍术多糖的工艺研究 孟利娜，许 静，南 楠，段 殷，宋晓娇，周 晶* 天津医科大学 药学院 天津市临床药物关键技术重点实验室，天津 300070 摘 要：目的 优化北苍术多糖的超声提取工艺。方法 利用超声波提取北苍术根茎中的多糖，以提取物得率和多糖提取率 为指标，在单因素试验的基础上，采用 4 个因素（超声时间、料液比、超声温度、乙醇用量）3 个水平的 L 4 9(3 )正交试验优 选超声提取工艺条件，并采用苯酚–浓硫酸法测定多糖。结果 北苍术多糖的最佳提取工艺条件为：料液比 1 ∶20 、提取温 度 60 ℃、超声提取 40 min 、浓缩后加入 4 倍量乙醇进行醇沉。在该实验条件下，苍术水提物得率为 23.16% ，多糖提取率 为 11.23%。结论 超声法提取苍术中多糖简便快捷，多糖提取率高，为北苍术多糖的开发利用提供了参考。 关键词：北苍术；多糖；超声提取；正交试验 中图分类号：R284.2 文献标志码：A 文章编号：1674 - 5515(2012)05 - 0457 - 04 Optimization of ultrasonic extraction process for polysaccharide from Atractylodes chinensis by orthogonal test MENG Li-na, XU Jing, NAN-nan, DUAN Yin, SONG Xiao-jiao, ZHOU Jing Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics, School of Pharmacy, Tianjin Medical University, Tianjin 300070, China Abstract: Objective To optimize the extraction technology of polysaccharide from Atractylodes chinensis using ultrasonic extraction method by orthogonal test. Methods Polysaccharide was extracted from A. chinensis by ultrasonic-assisted extraction method. The yield of crude polysaccharide of A. chinensis and extraction rate of polysaccharide were taken as indices. Based on single-factor 4 experiment, the ultrasonic-assisted extraction technology of polysaccharide from A. chinensis was optimized by L (3 ) orthogonal test. 9 The phenol-sulphuric acid method was applied for the determination of polysaccharide. Res