新生大鼠海马神经干细胞表达趋化因子受体CCR2的体外研究.pdfVIP

昆明 医学院学 报 2,008，(1)：32 35 CN 53—1O49l／R Journal ofKnnmin~Medical University 新生大鼠海马神经干细胞表达趋化因子受体CCR2的体外研究 丁 鹏 ”，薛黎萍 ，杨智勇”，王进昆”，余化霖”，冯忠堂 ) (1)昆明医学院第一附属医院神经外科，云南昆明 650032；2)云南省第二人民医院眼科， 云南昆明 650021；3)昆明医学院，云南昆明 650031) [摘要]日的 探讨趋化因子受体CCR2在体外培养神经干细胞的表达．方法 采用无血清方法分离、培 养新生大鼠海马神经干细胞，细胞免疫荧光方法检测神经干细胞标志巢蛋白 (nestin)表达及干细胞多向分化为 神经元及胶质细胞的能力，通过细胞免疫荧光及RT—PCR的方法检测神经干细胞表达趋化因子受体CCR2的情 况．结果 体外培养新生大鼠海马神经干细胞呈nestin阳性，可分化为NF200阳性神经元、GFAP阳性星形胶 质细胞及CNP阳性少突胶质细胞，CCR2细胞免疫荧光及RT—PCR证实神经干细胞表达趋化因子受体CCR2． 结论 新生大鼠海马神经干细胞表达趋化因子受体CCR2，为进一步研究其体内、外迁移提供理论依据． [关键词]神经干细胞；趋化因子；体外 [中图分类号】R329．2*8【文献标识码】A【文章编号】1003—4706(2008)0l一0032—04 In Vitro Study about Expression of Chemokine Receptor CCR2 in Neural Stem Cells of Neonatal Rat Hippocampi DING Peng”，XUE Li—ping ，YANG Zhi—yong”，WANG Jin—kun”，YU Hua-lin”，FENG Zhong—tang (1)Dept．ofNeurosurgery，The]st Affiliated Hospital ofKunming Medical University，Kunming Yunnan 650032；2)Dept．ofophthalmology，The 2nd People’S Hospital ofYunnan Province，Kunming Yunmm 650021；3)Kunming Medical University，Kunming Yunnan 650031，China) [Abstract]Objective To explore whether neural stem ceHs，in vitro，expressed chemokine receptor CCR2．Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in serum-flee medium．By using immunofluorescence，the neural stem cells and its differentiation into neuron and glial cell types were identified．Then immunofluorescence and RT-PCR were employed to detect whether neural stem cells，in vitro，expressed chemokine receptor CCR2．Results The floating neural spheres，isolated from neonatal hippocampi，were nestin positive and could differentiate into cells to express characteristic antigen of neurons，astrocytes and oligodendrocytes．Immunofluorescence and RT-PCR showed neural stem cells expressed chemokine recep