江门成人散发急性腹泻者星状病毒的检测及毒株型别分析.pdfVIP

• 420 • JoumA_of Tropical Medicine Vo1.8 No.5 Mav.2∞8 [文章编号1 1672-3619(2∞8)05-0420-03 ·硕博专栏论著· 江门成人散发急性腹泻者星状病毒的检测及毒株型别分析 徐巧华，唐亚丽，聂军飞蔡练功，李建栋，戴迎春，陈清，俞守义 (南方医科大学公共卫生与热带医学学院流行病学教研室，广州 510515) [摘要] 目的 研究也门地区成人腹泻者星状病毒感染情况及型别特点。方法 收集江门地区病毒性腹 泻流行期散发成人腹泻患者粪便标本，经胶体金法、RT-PCR 法分别检测轮状病毒和诺如病毒后，运用实时荧光 PCR 进行星状病毒检测，并对阳性标本进行 RT-ne由d PCR 测序，鉴定型别。结果 56 份成人病毒性腹泻粪便 标本中，荧光 PCR 法测出 3 份星状病毒株(5 .4%)，经 RT-PCR 测序 l 份，序列分析鉴定为 HAtsV-4 型。结论 江门成人散发病毒性腹泻者存在星状病毒感染，型别为 HAstV斗，未发现多致泻病毒混合感染。 [关键词】 成人;星状病毒;荧光 PCR; RT-nested PCR 中图分类号:R373 文献标识码:A De能ction and Genotyping of Astrovirus in Adults with Gastroenteritis in Jiangmen XU Qiao-hua , TANG Ya-li , NIE Jun* , CAI Lian-gong , U Jian-dong , DAI Ying-chun , CHEN Qi吨， YU Sh0117i (School 0/ Public He a1th and Tropica1 Medicine , Southem Medic a1 University , Guangzhou 510515 , China) [Abstract] Objective To study the pathogenic prevalence and genotypes of human astrovirus (HAtsV) in adult C酷es with viral diarrhea in Jiangmen , Guangdong province. Methods Adult c面白白白 sporadic viral diarrhea in Jiangmen were first screened for Rotavirus and Norwalk-like virus with Rotavirus Group A Dia喜nostic Kit (Collodial Gold Device) and reverse-transcription polymer困e chain reaction (RT-PCR) , respectively , and then followed by Real time TaqMan PCR for the detection of astrovirus. Astrovirus positive cases detected by TaqMan PCR were conformed by RT- nested PCR , and the DNA sequences of the amplified fr略me由were determined. Results A total of sixty five adult C皑白白白 a