表36批样品残留溶剂测定结果.PDFVIP

RP-HPLC 测定痛经宁胶囊中芍药苷的含量 江帆 1 2 1 ，郭培果 ，蔡晓静 (1.贵州师范大学分析测试中心，贵阳 550001 ；2.贵州科瑞特医药科技开发研究所，贵阳 550001) 摘要：目的 建立痛经宁胶囊中芍药苷的 HPLC 含量测定方法。方法 采用 Hypersil C18 柱(250 mm×4.6 mm ，5 μm) ，以 乙腈-0.1%磷酸(15 ∶85)为流动相；检测波长 230 nm ；流速：1.0 mL·min-1 ，柱温：30 ℃。结果 芍药苷在 0.256~1.536 μg 内线性关系良好，r=0.999 9 ，平均回收率为 99.49% ，RSD=0.56%(n=9) 。结论 该方法简便、准确、重复性好、可作为痛 经宁胶囊的含量测定方法。 关键词：痛经宁胶囊；芍药苷；反相高效液相色谱法 中图分类号：R917.101 文献标志码：B 文章编号：1007-7693(2010)08-0741-03 作者简介：江帆，女，硕士，主任药师 Tel: (0851) 6690018 E-mail: dyjiangfan@126.com 中国现代应用药学2010 年 8 月第 27 卷第 8 期 Chin JMAP, 2010 August, Vol.27 No.8 ·741 · Determination of Paeoniflorin in Tongjingning Capsules by RP-HPLC 1 2 1 JIANG Fan , GUO Peiguo , CAI Xiaojing (1.The Analysis and Testing Center in Guizhou Normal University, Guiyang 550001, China; 2.Guizhou kerui-te Institute of Pharmaceutical Science and Technology, Guiyang 550001, China) ABSTRACT: OBJECTIVE To establish a method for determining the concentration of paeoniflorin in Tongjingning capsules by RP-HPLC. METHODS RP-HPLC was used. Chromatography conditions: Hypersil C18 column(250 mm×4.6 mm, 5 μm), the mobile phase consists of acetonitrile-0.1% phosphoric(15 ∶85), the flow rate was 1.0 mL·min1, the detective wavelength was set at 230 nm, and column temperature was 30 ℃. RESULTS The calibration curve was linear in the range of 0.2561.536 μg for paeoniflorin(r=0.999 9). The average recovery of paeoniflorin was 99.46% and RSD was 0.56% (n=9). CONCLUSION The method is simple and accurate with good repeatability, which can be used for the quality control of Tongjingning capsules. KEY WORDS: Tongjingning capsules; paeoniflorin; RP-HPLC 痛经宁胶囊由当归、白芍、香附、延胡索、 0.25 g ，精密称定，置具塞锥形瓶中，精密加入稀 川芎、甘草、丹参、川楝子及红花等