纳克级激光计数检测器同时测定7种人工甜味剂 - 食品安全质量检测学报.doc

纳克级激光计数检测器同时测定7种人工甜味剂 (1. 中国食品药品检定研究院, 北京 100050; 2. 资生堂(中国)投资有限公司, 北京 100176) 摘 要: 目的 建立采用纳克级激光计数检测器(nano quantity analyte detector, NQAD)同时测定7种人工甜味剂的分析方法。方法纳克级激光计数检测器系统下, 使用CAPCELL PAK C18 MGII(150 mm×2.0 mm, 5 μm)色谱柱, 以20 mmol/L乙酸铵水溶液(A)-甲醇(B)为流动相进行梯度洗脱, 流速0.2 mL/min, 柱温40 ℃。结果7种常见人工甜味剂得到良好分离与检测, 在紫外检测器上难以检出的甜蜜素、三氯蔗糖和甜菊苷3种成分, 在NQAD检测器上分别得到了0.27、0.17、1.19 μg/mL的检出限。色谱峰面积精密度RSD＜4.97%; 标准曲线得到良好线性关系r20.994; 样品回收率96.69%~105.18%之间。结论使用新型NQAD建立了人工甜味剂安赛蜜、糖精钠、甜蜜素、三氯蔗糖、阿斯巴甜、纽甜、甜菊苷的高灵敏度共同分析方法, 方法简单、专属性高。 关键词 Simultaneous determination of 7 kinds of artificial sweeteners by nano quantity analyte detector HE Huan1, CAO Jin1*, YANG Qian2, GUO Yan-Li2* (1. National Institute of Food and Drug Control, Beijing 100050, China; 2. Shiseido China Co., Ltd.,Beijing 100176, China) ABSTRACT: Objective To establish a method for simultaneous determination of 7 kinds of artificial sweeteners by nano quantity analyte detector. Methods The samples were separated on a CAPCELL PAK C18 MGII column(150 mm×2.0 mm, 5 μm) with 20 mmol/L ammonium acetate solution (A)-methanol (B) as mobile phase at the gradient elution program. The flow rate was 0.2 mL/min and column temperature was 40 ℃. Results The 7 kinds of artificial sweeteners were separated completely with a C18 column, and detected by a NQAD. Molasses, sucralose and stevia which were difficult to be detected by UV could be detected by NQAD, and the limits of detection were 0.27, 0.17, 1.19 μg/mL, respectively. The RSDs of chromatographic peak areas were less than 4.97%, the correlation coefficients of 7 kinds of artificial sweeteners were more than 0.994, and the average recoveries were 96.69%~105.18%. Conclusion The NQAD can be used for the determination of 7 kinds of artificial sweeteners and this method is proved to be simple, accurate, sensitive and reliable. KEY WORDS: nano quantity analyte detector; artificial sweeteners; molasses; sucralose; stevia 1 引言 人工甜味剂可替代糖类物质添加到食品当中, 改善食品口感, 增加甜度, 具有高甜味、低热量、低成本等优点, 被广泛用于各种食品加工[1]。但人工