2005及2010年版《中国药典》肿节风中异秦皮啶含量测定方法比较.docVIP

2005及2010年版《中国药典》肿节风中异秦皮啶含量测定方法比较【摘 要】 目的：比较2005与2010年版《中国药典》肿节风中异秦皮啶含量测定方法的优劣。方法：分别按照2005和2010年版《中国药典》肿节风中异秦皮啶的样品处理方法处理同一批肿节风根、茎、叶样品，制备待测溶液，HPLC方法测定异秦皮啶的含量。结果：分别采用2005与2010年版《中国药典》的测定方法测得肿节风根、茎、叶中异秦皮啶平均含量分别为0.071 %，0.045 1%，0.025 3%和0.047 %，0.015 4%，0.0073%。结论： 2010年版方法测得异秦皮啶的含量显著低于2005年版方法，且灵敏度也显著低于2005版方法，建议对2010年版方法进行修订。 【关键词】 肿节风；《中国药典》；异秦皮啶；含量测定 Comparative Study on determination methods differences of 2005 and 2010 Year Edition Pharmacopoeiaof People’s Republic of China about Sarcandra glabra’s isofraxidine content SHI Ling-ling1, XIE Ru-gen2, ZHANG Yun-gen2, ZHANG Da-xing2, LIU Yu-jun1*, MA Chao1* (1. Beijing Forestry University, Beijing 100083, China;2. Jiang le National Forest Farm, Fujian 350401, China ) 【Abstract】Objective：To compare the methods in 2005 and 2010 Year Edition Pharmacopoeia of People’s Republic of China for determination of isofraxidine in Sarcandra glabra. Method: HPLC was used to measure the content of isofraxidine in the same samples of roots, stems and leaves of Sarcandra glabra, after samples preparation according to 2005 and 2010 Year Edition Pharmacopoeia of People’s Republic of China. Result: The average content of isofraxidine determined using the methods of 2005 and 2010 Year Edition Pharmacopoeia of People’s Republic of China in roots, stems and leaves was 0.071 8%, 0.045 1% , 0.025 3% and 0.047 1%, 0.015 4%, 0.007 3% respectively. Conclusion:The content of isofraxidine determined using 2010 Year Edition method was significantly much lower than 2005 Year Edition, and the methods in 2010 Year Edition was suggested to be modified. 【Key words】 Sarcandra glabra; Pharmacopoeia of People’s Republic of China; isofraxidine 肿节风（Sarcandra glabra）又名草珊瑚、九节茶、接骨木等，是金粟兰科（Chloranthaceae）植物草珊瑚Sarcandra glabra (Thunb.) Nakai的干燥全草。肿节风味苦、辛，性平，具清热凉血、活血消斑、祛风通络等功效，临床用于治疗血热紫斑、紫癜、风湿痹痛、跌打损伤等症[1]。药理学研究发现，肿节风具有抗癌、抗菌、抗病毒、抗炎镇痛等作用[2～7]。肿节风中含有的异秦皮啶在体外活性实验中显示出一定的抑制肿瘤细胞的作用[8]，因此常常将其作为肿节风质量控制的标识性成分。2000年版《中国药典》将异秦皮啶的含量作为草珊瑚药材及制剂的质量控制指标，200