金樱子对糖尿病鼠肝氧化应激影响.docVIP

金樱子对糖尿病鼠肝氧化应激影响【摘要】 目的 研究金樱子对链脲佐菌素(streptozotocin,STZ)和高糖高脂诱导的实验性糖尿病大鼠肝氧化应激的影响。方法 用STZ腹腔注射和饲喂高糖高脂诱导法建立SD大鼠糖尿病模型,随机分为糖尿病模型组和金樱子干预组,同时另设正常对照组和金樱子对照组。24周后处死大鼠,取肝组织,用免疫组化方法检测肝脏组织细胞MCP-1、NF-κB二个因子的表达。结果 糖尿病模型组MCP-1、NF-κB蛋白的表达与金樱子干预组比较有差异(P0.05)。结论 金樱子可以下调实验性糖尿病鼠肝MCP-1、NF-κB的表达,增强其抗氧化能力。 【关键词】 糖尿病;金樱子;肝脏;抗氧化作用 Effects of Rosa Laevigata Mickx on Expression of Oxidative Stress in Liver of Diabetic Rats XU Jin-hua,QIN Zhen-qi,ZHOU Yu-juan,et al. College of Pharmacy and Life Science, University of South China,Hengyang,Hunan 421001, China 【Abstract】 Objective To investigate the effects of Rosa Laevigata Mickx on oxidative stress in liver of STZ and high sucrose/high fat feed-induced model in rats with experimental diabetes.Methods The SD rats with diabetes by intraperitoneal injection of STZ and high sucrose/high fat feed were randomly divided into two groups : diabetes model group , and diabetes intervened with Rosa Laevigata Mickx.In addition another two groups were made : normal control group , control group with Rosa Laevigata Mickx.All groups were sacrificed at the 24th week,liver samples were collected.Protein expression of MCP-1 and NF-κB in liver was detected through immunohistochemistry.Methods The protein expression of MCP-1 and NF-κB in liver of the diabetes model group were higher than those of intervention groups (P0.05).Conclusion Rosa Laevigata Mickx can decrease expression of MCP-1 and NF -κB in liver of diabetes rats and increase antioxidation ability of diabetes rats. 【Key words】 Diabetes rats; Rosa Laevigata Mickx; Liver;Antioxidation 基金项目:湖南省科技厅资助项目(项目编号:06FJ3183) 作者单位:421001 南华大学药学与生命科学学院(许金华 秦振启 张秋菊); 南华大学护理学院(周钰娟);南华大学附属第二医院(罗玉平) 金樱子(Rosa Laevigata Mickx),又称为刺梨子、刺榆子,入药历史悠久。我们课题组研究发现,用含有金樱子的饲料喂养糖尿病大鼠后,血糖、血脂降低,并能减轻糖尿病时肾病的发生与发展[1]。本实验进一步研究金樱子抗氧化应激的作用机理,检测金樱子对实验性糖尿病大鼠肝脏的 IKK/IκB/NF-κB信号通路中下游单核细胞趋化因子-1(MCP-1)、核转录因子(NF-κB)表达的影响,为防治糖尿病提供新依据。 1 材料与方法 1.1 实验动物 90只雄性SD大鼠购自南华大学实验动物部(许可证:SYXK(湘)2004-0011),约2月龄,体重为(250 ±20) g,适应性饲养1周。