新型重组高效复合干扰素对卵巢癌细胞生长抑制及促凋亡作用.docVIP

新型重组高效复合干扰素对卵巢癌细胞生长抑制及促凋亡作用【摘 要】 目的:探讨新型重组高效复合干扰素对卵巢癌HO8019细胞的作用，并与同类型复合干扰素、普通重组Ⅰ型干扰素、化疗药物对比。方法：应用新型重组高效复合干扰素、复合α干扰素、干扰素α-2b和顺铂以0.156ug/ml、0.625ug/ml、2.5ug/ml及10ug／ml浓度作用于体外培养的HO8019细胞72小时后，MTT法检测并计算细胞抑制率并比较。应用新型重组高效复合干扰素、复合α干扰素、干扰素α-2b和顺铂以0.156 ug/ml、0.625 ug/ml、2.5 ug/ml浓度作用于体外培养的HO8019细胞72小时后，以流式细胞仪检测HO8019细胞的凋亡率并进行比较。结果：几种干扰素及顺铂均能导致HO8019细胞生长抑制及促进细胞凋亡。相同浓度条件下，新型重组高效复合干扰素和顺铂对HO8019细胞的抑制及凋亡作用强于复合α干扰素和干扰素α-2b的作用，尤以顺铂的作用最强。结论：重组高效复合干扰素对体外培养的卵巢癌HO8019细胞的生长有抑制作用，并能促进HO8019细胞凋亡；作用虽然不及化疗药物顺铂，但较同类型干扰素、普通重组Ⅰ型干扰素的作用强。 【关键词】 干扰素； 卵巢癌； 基因重组 The Growth Inhibition and Αpoptosis-promoting Effect OfrSIFN-co tothe Ovarian Carcinoma Cells in Vitro TANG Xiumei, HE Kai, GAO Dehong, LIU Bo The Fifth Affiliated Hospital of Qiai Haer Medical College, Heilongjiang 163454, China 【Abstract】 Objectives: To investigate the effect of rSIFN-co consensus Interferon，IFNa-2b(Interferon a-2b) and chemotherapeutics on ovarian cancer cell line HO8019 in vitro． Methods： Αfter HO8019 cervicαl cαncer cells were cultured in vitro with rSIFN-co、Infergen、IFNα-2b αnd cisplαtin(DDP)αt the concentrαtion of0.156ug／ml、0.625 pg/ml、2.5 ug/ml and 10 g/ml respectively for 72 hours，cell proliferαtion was mensurated by MTT assay.Cell inhibition rate was calculated.Then after HO8019 ovarian cancer cells were treated with rSIFN-co、Infergen、IFNa-2b and DDP at the concentration of 0.156 ug/ml、0.625 pg/ ml、2.5 pg/ml for 72 hours，cell apoptosis wαs determined by flow cytometry． Results： rSIFN-co、Infergen、IFNα-2b and DDP could induce inhibition and apoptosis of HO8019 cells.The inhibition and apoptosis of HO8019 cells which had been induced by rSIFN-co and DDP were better than which had been induced by Infergen and lFNα-2b at the same concentration，and DDP was more better than rSIFN-co. Coclusions： rSIFN-co has antineoplastic efect by inhibiting proliferation and inducing apoptosis in HO8019 cervica cancer cell in vitro． The effect is inferior to DDP，but better than the same type interferon and general type