流式细胞仪在B 细胞肿瘤诊断和治疗中的作用 - 肿瘤药学.PDFVIP

- 322 - 肿瘤药学 2012 年 10 月第 2 卷第 5 期　Anti-tumor Pharmacy, October 2012, Vol. 2, No. 5 ●综　　述● 流式细胞仪在 B 细胞肿瘤诊断和治疗中的作用 张丽凤 （宾夕法尼亚大学流式细胞术与细胞分选资源实验室，费城　宾州，19 104-6082 ，美国） 摘要：流式细胞仪免疫分型技术（flow cytometric immunophenotyping, FCI）现已成为 B 淋巴细胞肿瘤诊断和治疗 中不可缺少的工具，它结合了众多研究领域的新技术，具有快速、准确、灵敏等优点。该项技术可以鉴别处于不同成 熟阶段的 B 细胞及属于不同生物功能区的 B 细胞；通过检测轻链限制及异常抗原的表达区分肿瘤性 B 细胞和反应性 B 细胞并且将肿瘤细胞进行分型，分类等。近来，该技术通过检测白血病的微小残留、B 淋巴细胞肿瘤细胞特定表面 抗原的测定以及细胞内信号传递通路的变化，在 B 淋巴细胞肿瘤的复发评估、临床用药、药效评估中发挥了越来越 大的作用。 关键词：流式细胞仪；B 淋巴细胞瘤；诊断 中图分类号：R730.4　文献标识码 ：B　文章编号：2095-1264（2012）05-0322-010 doi：10.3969/j.issn.2095-1264.2012.05.001 The Role of Flow Cytometry in the Diagnosis and Treatment of B Cell Lymphoma Zhang Lif eng (Flow Cytometry and Cell Sorting Resource Laboratory, University of Pennsylvania, Philadelp hia, PA 19104-6082, USA) Abstract: Flow cytometric immunophenotyping (FCI) has become an indispensable approach for the diagnosis, classifica- tion, staging, and monitoring of B cell lymphoma. In conjunction with many other new techniques, this technique can be used to detect neoplastic cells with high sensitivity and specificity and it can be done within several hours. By using this technique, B-lymphocytes can be recognized by expression of lineage-associated antigens, the stages of maturation and belonging to dif- ferent biologic compartments; neoplastic B-cells can be distinguished from reactive B-cells by demonstration of immunoglobu- lin light chain restriction or phenotypic aberrancy; and the phenotype of the neoplastic cells can be used to identify one of the distinct disease entities. In addition, visualization of minimal residual disease, lymphoma speci