溶剂对挥发油包合工艺中包合率测定影响探究.doc

溶剂对挥发油包合工艺中包合率测定影响探究[摘要] 目的:研究不同的洗脱和提取溶剂对中药挥发油β-环糊精包合率测定的影响,建立准确的包合率测定方法。方法:以石菖蒲油包合物为模型,采用不同极性的溶剂分别进行包合物表面未包合游离药的洗涤,以及包合物中已包合药物的提取,筛选出合适的溶剂组合用于挥发油包合物的包封率测定。结果:不同极性的溶剂对石菖蒲油包合物的洗涤、提取效果有很大的差别,洗涤时药物损失由小到大为石油醚乙酸乙酯。结论:选择极性小的石油醚为洗脱溶剂、极性大的乙醇为提取溶剂,可以准确地测定挥发油包合物的包合率。 [关键词] β-环糊精包合物;包合率;有机溶剂;洗涤;提取 [中图分类号]R927 [文献标识码] A[文章编号] 1673-7210(2009)08(a)-080-02 Influence of elute and extraction solvent on the inclusion efficiency determination of essential oil and β-cyclodextrin complex HUANG Yu1, DU Feng2, HUANG Hai1, DU Song1 (1.National Project Research Center for the Modernization of Extraction and Separation of TCM/Guangzhou Hanfang Modern Medicine Research Development Co., Ltd, Guangzhou510240, China; 2.Heilongjiang Dongmei Group, Jiamusi154004, China) [Abstract] Abjective: To investigate the influnce of elute and extraction solvents on the determination of inclusion efficiency of β-cyclodextrin(β-CD) complex. Methods: Acorus tatarinowii Schott was used as model. Solvents of different polar were used for washing the uncomplexed oil and extracting the complexed oil respectively. The optimum solvent group were selected to determine the inclusion efficiency. Results: There existed big difference between solvents of different polar used for washing the Acorus tatarinowii Schott inclusion and extracting the mixture. Low polar solvent was fit for washing and high polar solvent was fit for extracting. Conclusion: The low polar solventpetroleum and high polar solvent ethanol are selected as elution and extraction solvent respectively for the inclusion efficiency determinationof essential oil β-CD complex. [Key words]β-cyclodextrin; Complex inclusion efficiency; Organic solvent; Washing; Extraction 挥发油是中药中常见的有效成分,在制备中药固体制剂时常采用β-环糊精包合技术,使挥发油与环糊精生成稳定的包合物,增加药物的稳定性,同时使液体药物粉末化,目前该技术已经广泛应用于含有挥发油的中药固体制剂工艺中[1-2]。 在包合物的制备研究中,人们通过测定包合率来考察、优选包合制备工艺。已有的关于挥发油包合的工艺文献中[3-5],包合率测定时采用的洗涤及提取的溶剂比较混乱,为此,本文在石菖蒲油β-环糊精包合物的研究过程中,对包合率测定中几种常用的溶剂进行了考察筛选,确定了准确测定包合率的洗涤及提取溶剂,现报道如下: 1 仪器与