溴氰菊酯对大鼠脑血红素氧化酶-1影响.doc

溴氰菊酯对大鼠脑血红素氧化酶-1影响[摘要] 目的：研究溴氰菊酯（DM）对大鼠脑皮质及海马血红素氧化酶-1（HO-1）mRNA及蛋白表达的影响。方法：健康SD雄性大鼠36只，随机分为对照组（腹腔注射色拉油）、低剂量DM组（腹腔注射1.25 mg/kg DM）、高剂量DM组（腹腔注射12.5 mg/kg DM），连续染毒5 d，末次染毒24 h后以原位杂交法测定皮质及海马HO-1 mRNA的表达，以免疫组化法测定HO-1蛋白表达。结果：与正常对照组相比，高剂量DM组HO-1 mRNA表达阳性细胞数明显增多（P＜0.05）；而HO-1蛋白表达阳性细胞数则在低剂量DM组、高剂量DM组大鼠均明显增高（P＜0.05），并随着染毒剂量的增加表达明显增强（P＜0.05）。结论：溴氰菊酯可诱导大鼠脑皮质及海马HO-1表达增加，发挥一定保护作用。 [关键词] 溴氰菊酯；血红素氧化酶-1；大鼠 [中图分类号] R91 [文献标识码]B [文章编号]1673-7210（2008）03（c）-024-02 The effects of deltamethrin on heme oxygenase-1 in rats LIU Yu-mei，WU Ling，WANG Ji-hong，YI Cong (Department of Preventive Medicine, Hainan Medical College, Haikou 571101,China) [Abstract] Objective: To observe the expression changes of heme oxygenase-1(HO-1) and its mRNA induced by delmathrin（DM） in rats. Methods: 36 healthy male SD rats were randomly divided into control, low DM（1.25 mg/kg DM）, high DM（12.5 mg/kg DM）exposed groups, by giving intraperitoneal injection for 5 days. The number of protein expression of hemeoxygenase-1 and its mRNA were detected at 24 hours after the last intraperitoneal administration.Results: The HO-1 and its mRNA expressed positive cells were significantly increased in high DM group (P 本研究结果显示，腹腔注射低、高剂量溴氰菊酯后，大鼠皮质及海马HO-1蛋白表达均明显上调，并随染毒剂量的增加而增强。HO-1 mRNA的表达则在高剂量组上调明显，而在低剂量组变化不明显，这提示，溴氰菊酯可诱导脑HO-1表达上调，蛋白水平的变化较mRNA敏感，较低剂量即可引起HO-1蛋白表达的显著增强。但HO-1是否在溴氰菊酯的神经损伤中发挥保护作用及其机制如何、程度多大，还有待进一步研究。 [参考文献] [1]Wu A, Liu Y.Deltamethrin induces delayed apoptosis and altered expression of p53 and bax in rat brain[J]. Environ Toxicol Pharmacol，2000, 8:183-189. [2]李涛,石年,钟玉芳等.溴氰菊酯对大鼠脑神经细胞凋亡及Caspase-3表达的影响[J].中华劳动卫生职业病杂志,2004,22(5):371-374. [3]贺锡雯,吕京.有机磷类和拟除虫菊酯类的神经毒作用机理[J].中国药理学与毒理学杂志,1997,11(2):89-90. [4]牛玉杰,石年,严红,等.拟除虫菊酯对大鼠神经细胞凋亡的诱发作用[J].卫生毒理学杂志,1998,12(4):210-214. [5]Foresti R, Motterlini R. The heme oxygenase pathway and its interaction with nitric oxide in the control of cellualr homeostasis[J]. Free Radic Res, 1999, 31(6):459-475. [6]Panahian N, Yoshiura M, Maines MD. O