电针对脑缺氧缺血新生大鼠海马结构Nestin及Brdu表达影响.doc

电针对脑缺氧缺血新生大鼠海马结构Nestin及Brdu表达影响[摘要] 目的：通过观察电针对脑缺氧缺血新生大鼠海马结构中神经上皮干细胞蛋白(Nestin)与5-溴-2-脱氧脲嘧啶(Brdu)表达的影响，了解电针对脑缺氧缺血后神经发生水平的干预，为电针穴位疗法的临床应用提供实验依据。方法：100只7日龄健康Wistar大鼠，体重12～15 g ，随机分为4组：空白对照组、模型判定组、缺氧缺血组与缺氧缺血后电针治疗组，各组动物(除空白对照组外)分别结扎左侧颈总动脉，然后将动物置于8%O2-92%N2混合气中2 h 后取出。模型判定组动物在缺氧缺血48 h后处死，制备脑组织切片。脑缺氧缺血后电针组在缺氧缺血后恢复2 d，开始刺激“百会”和“大椎”两穴。24 d后除模型判定组外，各组动物分别取左侧脑组织制作标本，行Nestin与Brdu免疫组化染色。结果：脑缺氧缺血后电针组海马CA1区Nestin免疫阳性细胞数高于正常对照组及脑缺氧缺血组(P＜0.05)。脑缺氧缺血后电针组齿状回Brdu免疫阳性细胞数高于正常对照组及高于脑缺氧缺血组(P＜0.05)。结论：电针穴位治疗可明显提高(加强)脑缺氧缺血后海马结构神经发生的水平(能力)。 [关键词] 电针；缺氧缺血；Nestin；Brdu [中图分类号] R246.6[文献标识码]A [文章编号]1673-7210（2008）09（c）-009-03 The influence of electroacupuncture on the expressions of Nestin and Brdu in the hippocampal formation of neonatal rat brain with hypoxia-ischemia ZHOU Xiu-li1, FAN Wei-min2 (1.Department of Neurology, Xinyang Central Hospital of Henan Province, Xinyang 464000, China; 2.Department ofPhysiology, Xinyang Vocational and Technical College, Xinyang 464000, China) [Abstract] Objective: To observe the influence of electroacupuncturing on the Nestin and Brdu expressions in the hippocampal formation of neonatal rats with hypoxia-ischemia, and to confirm the effect of electroacupucture(EA) on HIBD (Hypoxic-ischemic brain damage) in neonatal rats, and then provide the theoretical basis for clinical application. Methods:7 days old Wistar rats(n=100), weighing 12-15 g, were randomly divided into four groups: the control group, the model group, HIBD group and electroacupucture(EA) group. Except the control group, the other three groups were prepared by left common carotied artery ligation and systemic hypoxia (8% oxygen in nitrogen) for 2 h. Then the rats in model group were sacrificed. After 2 d of recovery, the rats in electroacupucture group were treated with electroacupucture (“baihui” and “dazhui”points) every day till the rats were sacrificed 22 d after HIBD. The rest rats were subjected to perfusion-fixation 22 d later, the left brains were regularly paraffin wax e