祛风巴布剂中青藤碱含量测定.docVIP

祛风巴布剂中青藤碱含量测定[摘要] 目的：建立祛风巴布剂中青藤碱含量的测定方法。方法：色谱柱为Thermo Hypersil-Keystone C18(250 mm×4.6 mm，5 μm)。流动相为A（甲醇∶乙腈∶二乙胺=18∶14∶0.5）-B（水∶二乙胺=58∶0.6）为40∶60，柱温为25℃，流速为1.0 ml/min，检测波长为262 nm。结果：青藤碱在2.862×10-2～1.118 mg/ml范围内，浓度与峰面积有良好的线性关系。平均回收率为100.90%，RSD=1.23%。三批样品的平均含量为59.726 mg/片。结论：经方法学验证，本测定方法专属性强，精密度、重现性等均较好，可用于祛风巴布剂中青藤碱的含量测定。 [关键词] 祛风巴布剂；青藤碱；含量测定 [中图分类号] R927.2[文献标识码]A [文章编号]1673-7210（2008）08（c）-044-02 Content determination of sinomenine in Qufeng Cataplasm WANG Yi (The First Affiliated Hospital, Heilongjiang University of Chinese Medicine, Harbin150040, China) [Abstract] Objective: To establish the method for the content determination of sinomenine in Qufeng Cataplasm. Methods: The column was Thermo Hypersil-Keystone C18(250 mm×4.6 mm, 5 μm) ,the mobile phase was A(methanol∶acetonitrile∶diethylamine=18∶14∶0.5) -B(water∶diethylamine=58∶0.6) (40∶60), the column temperature was 25℃, flow rate was 1.0 ml/min, and the detective wavelength was 262 nm. Results:There was a good linearity of sinomenine in the range of 2.862×10-2~1.118 mg/ml. The average recovery was 100.90% with RSD of 1.23%. The average content of sinomenine for three batchs samples was 59.726 mg/tablet. Conclusion:The method established in this article has good specificity, precision, reproducibility, and it can be used for the content determination of sinomenine in Qufeng Cataplasm. [Key words] Qufeng Cataplasm; Sinomenine; Content determination 巴布剂是以水溶性高分子聚合物为基质骨架材料的外用贴剂，与传统的黑膏药、现代的橡胶膏相比，具有保湿、透气、耐老化、载药量大、刺激性小、过敏性小的优点，是中药外用贴剂的适宜剂型。青藤碱（Sinomenine）存在于防已科植物青藤（Sinomenium actum Rehd.et wils.）的根和茎，蝙蝠葛（Menispermun dauricum DC.）叶等。具有肯定的镇痛镇静、镇咳局麻、降血压、抗感染作用，为植物中很强的组织胺释放剂。动物实验提示在休克和器官损害中有保护作用，临床上用于治疗风湿性关节炎和神经痛。本文对中药祛风巴布剂中青藤碱的含量进行了HPLC法测定。 1仪器与试药 Agilent 1100HPLC高效液相色谱（美国Agilent公司）；HB-1730QT超声波清洗机（深圳恒波超声设备有限公司）。中药祛风巴布剂（自制，20 cm×12 cm，批号：060901，060902， 060903）；青风藤对照品（中国药品生物制品检定所提供，批号：110774-200206）。甲醇、乙腈、二乙胺均为色谱纯(保定明发生物科技有限公司，批号：080305)；水为蒸馏水。 2方法与结果 2.1色谱条件 色谱柱为：Thermo Hypersil-K