解毒祛瘀滋阴方对MRL-lpr小鼠粘附分子表达影响.docVIP

解毒祛瘀滋阴方对MRL/lpr小鼠粘附分子表达影响[摘要] 目的 探讨解毒祛瘀滋阴方可能的作用机制。方法 以MRL/lpr小鼠80只,分成4组采用RT-PCR技术检测各组肾组织ICAM-1和VCAM-1的mRNA表达水平。结果 解毒祛瘀滋阴方合用激素组ICAM-1和VCAM-1mRNA表达低于其他各组。结论 解毒祛瘀滋阴方合用激素能有效降低MRL/lpr小鼠肾组织ICAM-1和VCAM-1mRNA表达水平。 [关键词] ICAM-1;VCAM-1 [中图分类号] R25 [文献标识码] A [文章编号] 1673-9701(2010)06-22-02 Effect of Detoxification-Stasis Removing-Yin Nourishing Recipe on mRNA Expression of ICAM-1 and VCAM-1 in MRL/lpr Mice YANG Kepeng1FAN Yongsheng2WANG Xinchang2WEN Chengping2 1.Zhengzhou Hospital of Traditional Chinese Medicine,Zhengzhou 450007,China;2.The China university of Zhejiang,Hangzhou 310053,China [Abstract] Objective To study the effect of detoxification-stasis removing-Yin nourishing (DSY) recipe on the mRNA expression of ICAM-1 and VCAM-1 in renal tissue in MRL/lpr mice. Methods We divided 80 MRL/lpr mice into four groups and used RT-PCR to detect the mRNA expression levels of ICAM-1 and VCAM-1 in the mouse renal tissue in each group. Results The mRNA expression levels of ICAM-1 and VCAM-1 in the mouse renal tissue were lower in the DSY recipe plus hormone group than those in other groups. Conclusion The DSY recipe plus hormone can effectively reduce the mRNA expression of MRL/lpr mouse kidney tissue ICAM-1 and VCAM-1. [Key words] ICAM-1;VCAM-1 系统性红斑狼疮(systemic lupus erythematosus,SLE)是一种典型的自身免疫性疾病,在我国的患病率约为75/10万人[1]。解毒祛瘀滋阴方是范永升教授治疗系统性红斑狼疮临床经验的结晶,前期研究表明,能有效调节机体细胞因子及其受体、新喋呤水平、影响淋巴细胞凋亡及Fas基因表达等[2-4]。 1材料与方法 1.1实验动物 MRL/lpr狼疮小鼠,雌性,7～8周龄,浙江中医药大学动物实验中心购进。 1.2实验药品 中药复方煎剂,由蚤休15g、青蒿30g、赤芍10g、积雪草15g、生地18g、炙鳖甲10g等12味解毒、祛瘀、滋阴药配伍而成,水煎,浓缩成生药含量1.50g/mL,由浙江中医药大学制剂室提供。强的松悬液,由强的松片溶于生理盐水按含药量0.5mg/mL配制而成。 1.3主要实验器材 RT-PCR试剂盒购自上海捷兰生物有限公司;紫外分光光度计 PharmaciaBio公司,瑞典;PCR温控仪Biometra公司,美国;Tanon 2000紫外分析仪及凝胶图像分析系统,上海天能公司。 1.4实验方法 1.4.1分组与用药随机将80只MRL/lpr小鼠分成4组,20只/组。模型组灌胃生理盐水;中药组灌胃中药解毒祛瘀滋阴浓缩液;西药组灌胃强的松混悬液;中西药组中西药隔日交替灌胃,均约0.8mL/日。用药10周后处死,冰上分离双侧肾脏并迅速置液氮中冻存。 1.4.2肾组织ICAM-1、VCAM-1mRNA表达检测(RT-PCR法)①引物设计由上海生物工程技术服务有限公司合成。引物序列:ICAM-1上游引物CATCCCAAAGCTCGACACCC(732位