生防枯草芽孢杆菌胞外植酸酶对小麦耐盐性的影响-researchgate.docVIP

生防枯草芽孢杆菌胞外植酸酶对小麦耐盐性的影响* 郭 英 刘 栋 赵 蕾﹡﹡ (山东师范大学生命科学学院 济南 250014) 摘 要 以产植酸酶的广谱拮抗生防枯草芽孢杆菌(Bacillus subtilis) T2为出发菌株，经硫酸二乙酯(DES)诱变，得到遗传特性稳定的一株植酸酶负突变株M3。采用有效磷限量、含NaCl 150 mmol L-1的植物生长水培液对小麦进行盐胁迫，在植酸存在的条件下，水培液中施加T2发酵液或植酸酶均可促进盐胁迫下小麦幼苗的生长，小麦株高、鲜重、叶片叶绿素含量和根系活力均得以增高，而植株丙二醛(MDA)含量降低。失去植酸酶活力的负突变株M3发酵液则不具有提高小麦株高鲜重和叶绿素含量的作用，对根系活力的提高和对MDA含量的降低程度也均低于T2发酵液处理。上述结果表明，生防芽孢杆菌胞外植酸酶在增强小麦耐盐性方面起了重要作用。图6表2参13 关键词 枯草芽孢杆菌；胞外植酸酶；诱变；小麦；盐胁迫（请注意关键词的规范性、科学性、全面性，以便相关人员的查询） CLC S482.8 : Q948.113？ Effects of Extracellular Phytase Produced by a Biocontrol Strain of Bacillus subtilis T2 on Salt Tolerance of Wheat Seedlings* GUO Ying, LIU Dong ＆ ZHAO Lei﹡﹡ (College of Life Sciences, Shandong Normal University, Jinan 250014, China) Abstract Strain Bacillus subtilis T2 was a potential biocontrol agent against a broad-spectrum plant pathogenic fungi with the ability to produce extracellular phytase. To evaluate whether the phytase secreted by T2 can enhance the saline tolerance of plant, mutagenetic program was undertaken for the construction of extracellular phytase derivates. A phytase minus mutant M3 with genetic stability was obtained by means of DES treatment. The results from biochemical experiments revealed that the culture filtrates of wild-type strain T2 with phytase activity and purified phytase stimulated growth of wheat seedlings under phosphate limitation in the presence of 150 mmol·L-1 NaCl and phytate. Significant increases in plant height, fresh weight, total leaf chlorophyll content and TTC reducing power and decrease in content of MDA were observed in response to addition of phytase and culture filtrates of T2 to culture liquids. However, the culture filtrates obtained from phytase-negative mutant strain M3 did not stimulate wheat seedlings growth under salt stress. These may provide strong evidence that phytase activity of B. subtilis is important for the increasing resistance of wheat to salt stress under phosphate limitation. Fig 6, Tab 2, Ref 13 Keywords Bacillus subtilis T