致病性大肠杆菌致树鼩肺炎死亡的诊断分析-中国试验动物学报.doc

从临床肺炎死亡树鼩中分离到致病性大肠杆菌 高家红，仝品芬，孙晓梅，江勤芳，代解杰 (中国医学科学院/北京协和医学院医学生物学研究所，云南省重大传染病疫苗研发重点实验室，昆明 650118) 【摘要】 目的 对例9种药敏试验，初步确定树鼩肺部感染的致病菌及其药敏情况。 结果 样本革兰氏染色见到大量阴性杆菌，抗酸染色结果显示为非结核分枝杆菌，大小约为0.2μm×2～6μm。营养琼脂培养6例样品中均仅见1株旺盛生长的细菌，进一步分离培养经革兰氏染色为阴性杆菌，抗酸染色为非结核分枝杆菌，大小和染色结果与样本涂片相同，经鉴定为致病性大肠杆菌。药敏试验表明该菌对头孢哌酮，呋喃妥因，氨苄西林，阿米卡星，氧氟沙星，诺氟沙星，磺胺甲噁唑/甲氧苄定高度敏感；对庆大霉素和青霉素G为低度敏感。 结论 6例树鼩死亡原因均为细菌性肺炎，病原菌初步鉴定为致病性大肠杆菌。药敏实验筛选出的药物可为临床治疗树鼩该类病例用药提供指导。 【关键词】 树鼩；致病性大肠杆菌；药敏试验 【中图分类号】R33 【文献标识码】A 【文章编号】 Isolating Enteropathogenic E.coli from Clinical Pneumonia in Dead Tree Shrews GAO Jia-hong, TONG Pin-feng, SUN Xiao-mei, JIANG Qin-fang, DAI Jie-jie (Institute of Medical Biology, Chinese Academy of Medicine Sciences(CAMS) Peking Union Medical College(PUMC), Yunnan Key Laboratory of Vaccine Research Development on Severe Infectious Diseases, Kunming 650118, China) 【Abstract】Objective To isolate, culture and identify the pathogenic bacterium from 6 cases of bacterial pneumonia within 1 month in tree shrews. Methods We Dissected the dead tree shrew, cut open the lung tissue with sterile blade to insert focus of infection in the lung with inoculating loop to get samples for inoculating on nutrient agar and another two samples for bacterial smear __________________________________________ [基金项目] “十一五”国家科技支撑计划项目 (项目编号：2009BAI83B02、2011BAI15B01)。 云南省科技基础条件平台建设计划项目（编号：2006PT07-2）资助。 [作者简介] 高家红（1968-），男，硕士，助理研究员，研究方向：实验动物学。Email:gjh@。 [通讯作者] 代解杰（1961-），男，研究员，项目负责人。E-mail：djj@ with Gram staining and acid-fast staining. The bacterium obtained was isolated and cultivated for bacterial growth observation, then the pathogenic bacterium of tree shrew and its antibiotic susceptibilities was initially identified by Gram staining, acid-fast staining, oxidase test, biochemistrical encoding Identification system and 9-kind antibiotic susceptibility test. Results A amount of negative bacteria were observed from samples with Gram staining. Acid-fast staining appears it was nontuberculous mycobacterium about size 0.2μm×