急性白血病免疫分型临床应用.docVIP

急性白血病免疫分型临床应用[摘 要] 目的 研究急性白血病细胞抗原表达特点及临床意义。 方法 选用12种单克隆抗体，用流式细胞仪对71例临床诊断为急性白血病患者进行免疫分型。 结果 急性髓性白血病（AML）患者绝大部分表达两种以上的髓系抗原，其中部分表达淋系抗原（CD7）。急性淋巴细胞白血病（ALL）患者均表达淋系抗原，并且将ALL分为T-ALL和B-ALL，两系抗原互不交叉表达，部分表达一种或两种髓系抗原。单核细胞白血病M5种，M5a CD14表达平均值为21.01%，M5b CD14表达平均值为62.61%。4例由慢粒急变为ALL患者均表达髓系抗原。在AML中，CD34表达阳性率为55.2%（21/38），在ALL中表达阳性率为35.7%（10/28）。 结论 在AML中，免疫分型是对传统的形态学分型较有价值的补充，在对混合性白血病的诊断中有确诊的价值，在表示ALL的分型中的价值优于传统的形态学分型。 关键词：流式细胞仪 急性白血病 免疫分型 The Clinical Application of Acute Leukemias immunological typing SHI Zhongrui1 ZHU Jie2 (1 ：laboratory of medical science, Affiliated Hospital of Medical Colleage of the Chinese People’s Armed Police Forces, Tianjin 300162 2.Department of Laboratory, the Second Affiliated Hospital of Dalian Medical University , Dalian 116027) [Abstract] Objective To study acute leukemia cell?s antigen expression charateristics and its clinical significance. [Methods] The immunological type of 71 case of acute leukemia patients were detected by flow cytometer with 12 monoclonal antibodies. [Results] Most of AML patients expressed Above 2 kinds of myeloid antigens, some of them expressed CD7. All ALL patients expressed lymphoid antigens. They were divided into T-ALL and B-ALL. There no cross expression between two lines of antigens, some of them expressed one or two kinds of myeloid antigens. In M5a average expression of CD14 was 21.01%. In M5b, average expression of CD14 was 62.61%. All of 4 case of ALL transformed from CML expressed myeloid antigen. In AML, positive rate of CD34 expression was was 55.2%(21/38). In ALL, Positive rate of CD34 expression was 35.7%. [Conclusion] In AML, immunological typing is valuable compensation for the classical morphological typing. It helps in the exact diagnosis of missed leukemia, which suggests that immunological typing value is bigger than classical morphological typing. [Key Words] Flow cytometer Acute leukemia Immunological typing 中图分类号：R557 文献标示码：B 文章编号：1005-0019（2008）3-0018-03 传统的白血病分型都是以形态学为基础