耳针及六味地黄丸加味预防及治疗去势雌鼠骨质疏松实验探究.docVIP

耳针及六味地黄丸加味预防及治疗去势雌鼠骨质疏松实验探究(贵阳医学院附属医院,贵州550004) [摘 要] 目的:探讨耳针和中药六味地黄丸加味对绝经后骨质疏松症的防治作用及其作用机理。方法:取66只SD雌性大鼠按体重随机分为6组:假手术组、模型组、耳针组、中药组、耳针十中药组、西药组。后4组分别经耳针、六味地黄丸加味、耳针加中药联合应用以及雌二醇治疗3个月,观察耳针及中药对去势雌鼠的影响。? 结论:耳针、中药皆能提高去势雌鼠的BMC、BMD,防治PMO。 [主题词] 阉割/骨质疏松绝经后/预防和控制;耳针;六味地黄汤/治疗应用ExperimentalStudyonPreventionandTreatmentofOsteoporosisinOvariectomized RatswithAuri?cularAcupunctureandModifiedLiuweiDihuangPillLuoXiaoguangLiuGuozhong,HeZhigu ang,etal(GuiyangMedicalCollege,Guizhou550004) [Abstract] Purpose ToexplorepreventionandtreatmentactionsofauricularacupunctureandmodifiedLiuweiDi?huangPillonpostmenopausalosteoporosisandtheirmechanisms.Methods Sixty?sixfemaleratswererandomlydividedintosixgroupsaccordingtotheirbodyweight,pse udooperationgroup(POG),modelgroup(MG),auricularacupuncturegroup (AAG),Chineseherbgroup(CHG),auricularacupunctureplusChineseherbgroup(AHG)andestrogengroup(EG).ExceptforthePOG,allthegroupswereovariectomized.Aftertheoperation ,AAG,CHG,AHGandEGweretreat?edrespectivelywithauricularacupuncture,LiuweiDihuangPill(LWP),auricularacupuncturecombinedwithLiuweiDi?huangPill,andestradiolbenzoas.Threemonthslater,theeffectsofauricularacupu nctureandLiuweiDihuangPillontheratswereobserved.Results BothauricularacupunctureandLWPcanpromoteBMCandBMDinovariectomizedrats,soastop reventandtreatPMO.Themechanismmaybeincreaseserumestrogenlevel,decrea seserumPTHlevelandraiseCTlevel,inhibitingboneresorption,increasingbonereformatio nanddecreasingbonereabsorptionandthenadjustingim?balanceofbonemetabolism.? [Keywords] Castration,Osteoporosis,Postmenopausal/prev;AuricularAcupuncture;LiuweiDihuan gDecoction/theruse 绝经后骨质疏松症(PostmenopausalOsteoporo?sis,PMO)是绝经后妇女的易患疾病。随着经济的发展和人口寿命的延长,这一疾病亦呈上升趋势。雌激素替代疗法(HRT或ERT)是预防和治疗PMO的首选方法,但服药时间长(5～10年)[1],并有一定副作用和危险性,如引起子宫内膜增生,子宫内膜癌和乳腺癌等[2,3]。因此,寻找能够防治PMO,经济简便,可以长期应用的药物和方法,具有重要的经济效益和社会效益。本实验采用切除6～8月龄SD雌性大白鼠双侧卵巢(即去势雌鼠)建立PMO模型,观察耳针和中药六味地黄丸加味对去势雌鼠PMO的影响,探讨耳针和中药六味地黄丸加味对PMO的预防和治疗作用及作用机理,并寻求能够替代HRT治疗PMO的可能途径。? 1 材料和方法 1.1 实验动物的分组和造模 (1)分组:6