高压氧对局灶性脑缺血再灌注大鼠脑组织小窝蛋白 - 中国康复医学杂志.pdf

ChineseJournalofRehabilitationMedicine, Jun. 2011, Vol.26,No.6 ·基础研究· 高压氧对局灶性脑缺血再灌注大鼠脑组织 小窝蛋白-1、基质金属蛋白酶-9 表达的影响* 赵 红 张倩茹 卢晓梅 张海鹏 陈学新1 1 2 2 3 摘要 目的：研究高压氧（HBO）对局灶性脑缺血再灌注大鼠脑组织小窝蛋白-1（Cav-1）、MMP-9表达的影响。 方法：雄性Wistar大鼠，随机分成假手术组、脑缺血再灌注组(IR)、IR+HBO、HBO组。复制局灶性脑缺血再灌注模 型。HBO和IR+HBO组经0.25MPaHBO治疗5次。采用比色法、免疫组化法及Western 印迹法检测血脑屏障的通透 性和Cav-1、基质金属蛋白酶-9（MMP-9）蛋白的表达。 结果：IR组第4、12、24、48、72小时脑组织伊文思蓝(EB)的含量与第0小时组相比明显增加，再灌注后第4小时EB的 含量最高。IR+HBO组脑组织EB的含量明显低于IR组。IR组第4、12、24、48、72小时 Cav-1、MMP-9蛋白表达明 显高于0小时组。IR+HBO组与IR组相比脑组织Cav-1、MMP-9蛋白表达显著减低。 结论：HBO降低了脑缺血再灌注时增高的脑微血管内皮细胞Cav-1蛋白和MMP-9的表达。这可能是HBO对脑缺 血再灌注时血脑屏障通透性起保护作用的机制之一。 关键词 高压氧；脑缺血再灌注；小窝蛋白-1；基质金属蛋白酶-9 中图分类号：R743.3，R459.6 文献标识码：A 文章编号：1001-1242（2011）-06-0550-05 Effects of hyperbaric oxygen on caveolin-1 and matrix metalloproteinase-9 in brain tissues after cere⁃ bral focal ischemia-reperfusion in rats/ZHAO Hong, ZHANG Qianru, LU Xiaomei, et al.//Chinese Jour⁃ nal of Rehabilitation Medicine, 2011, 26(6): 550—554 Abstract Objective: To investigate the effect of hyperbaric oxygen (HBO) on caveolin-1(Cav-1) and matrix metalloprotein- ase-9(MMP-9)inbraintissues after cerebral focalischemia-reperfusioninrats. Method: Male Wistar rat were randomly assigned into sham, ischemia-reperfusion (IR), HBO, and IR+HBO groups. On cerebral focal ischemia-reperfusion models, 0.25MPa HBO were applied 5 times, and 2% Evens blue (EB) was injected into tail veins 1h before execution. Expressions of Cav-1 and MMP-9, contents of EB in brain tissuesweredeterminedby Westernblottechnique, immunohistochemistry,and spectrophotometer,respectively. Result: In IR group, EB content in brain tissues increased significantly compared with 0h, reached its peak at the 4th h(P0.01). EB content in IR+HBO group was significantly lower than that in IR group. While expressions