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药敏试验介绍(Introduction of drug sensitivity test)
药敏试验介绍(Introduction of drug sensitivity test)
Introduction of drug sensitivity test
Antibiotics to control bacterial infectious disease plays a very important role, but because of the breeding process is not scientific and blind drug abuse, many pathogenic bacteria develop resistance, the control effect of antibacterial drugs and bacterial disease worse, not only caused the waste of medicine, but also delayed diagnosis. It caused great economic losses to farmers. With the emergence of new pathogens, the efficacy of antimicrobial agents is getting worse and worse. Moreover, the sensitivity of various pathogenic bacteria to different antimicrobial agents is different, and the sensitivity of different strains of the same bacterium to different antimicrobial agents is also different. For a long time, drug resistance of pathogenic bacteria of various antimicrobial agents often lose efficacy, and do not have a good grasp of the drug sensitivity of bacteria, so a correct result for clinicians to select antibiotics reference, and improve the curative effect. The animal disease investigation and treatment center of Qingdao Yi state Biological Engineering Co., Ltd. of the Ministry of agriculture, animal husbandry and Quarantine Institute has summed up several sets of methods for drug sensitivity test at the grass-roots level.
The classification of antimicrobial susceptibility test
Disk diffusion method
This method is the quantitative filter paper containing antibacterial drugs have been posted on the inoculated agar surface test bacteria and drug susceptibility in agar diffusion, with diffusion distance increased, antibiotic concentration showed a logarithmic decrease, thus forming a concentration gradient on the disk around. At the same time, were not paper around inhibitory concentration range of growth, the two strains can be outside the scope of antibacterial growth, thereby forming a transparent disk in bacteriostatic ring around the diameter of inhibition zone of differ
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