空气负氧离子抑制肿瘤的研究--关于森肽基负氧离子疗效的临床实验.docVIP

空气负氧离子抑制肝癌细胞的实验研究 —关于森肽基生态级负氧离子生成机的实验研究 陶名章1(，李慧2，陈少周3，李海涛4 （1．中国空气负离子暨臭氧研究学会，广州510300；2. 南方医科大学附属南方医院呼吸睡眠中心，广州510516;3.香港玛丽医院精神科，香港999077；4.中山大学附属第一医院神经内科，广州510080） 【摘要】目的 研究空气负氧离子对肝癌Hepa1-6细胞生物活性的影响。方法 把细胞随机分为3组：空白对照组，空气对照组和负氧离子治疗组。治疗组根据负氧离子作用时间分为1h组、2h组和4h组。对细胞进行Tunel染色检测凋亡情况，流式细胞仪检测细胞周期变化情况，以WesternBlot法检测磷酸化P38MAPK的表达变化。结果 与空白对照组和空气对照组比较, 空气负氧离子治疗组肝癌细胞凋亡率明显增加,处于S期肝癌细胞所占有的比例下降明显, 磷酸化P38MAPK 表达上升明显, 差异均具有统计学意义(P 0.05)。结论 空气负氧离子通过阻滞细胞周期使肿瘤细胞发生凋亡, 进而抑制肿瘤细胞，该效应是通过P38MAPK信号通路传导来实现的。 【关键词】空气负氧离子，空气负离子，肝癌，P38MAPK Study of Negative oxygen ions inhibiting liver cancer cells in vitro TAO Ming-zhang1，LI Hui2,CHEN Shao-zhou3,LI Hai-tao4 (1.China on Negative Air Ions and Ozone Reseach Society，Guangzhou 510300，China；2. Center of Respiration and Sleep，Nanfang Hospital Affiliated Nanfang Medical College，Guangzhou 510516，China；3. Department of Psychiatry，Hongkong Queen Mary Hospital，Hongkong 999077，China；4. Department of Neurology，First Hospital Affiliated SUN YAT-SEN University，Guangzhou 510080，China ) 【Abstract】Objective：To investigate the affect on the biologic activity of hepatoma Hepa1-6 cell line of negative oxygen ions in vitro. Methods Dividing the cell into three groups: negative control group, air control group and treatment group，randomly.Dividing the treatment group into 1h、2h4h group according to action time of negative oxygen ions, all the cells in each group were detected apoptosis and cell cycle with flow cytometry method, the phospho- P38MAPK expression was detected. by Westernblot assay. Results：The apoptosis rate increased, the proportion of S stage cell decreased and the expression of phospho- P38MAPK increase obviously vs negative and air control group, the difference show significance ( P 0.05). Conclusion：The negative oxygen ions can induce hepatoma Hepa1-6 cell apoptosis through arresting the cell cycle, such effect was mediated by P38MAPK pathway. Key words: Negative oxygen ions; Negative air ions; Hepatoma; P38MAPK 被誉为空气维生素的空气负氧离子对人体健康十分有益。研究表明，