外源性一氧化碳释放分子对脓毒症小鼠肺部炎症反应的抑制作用.doc

　　外源性一氧化碳释放分子对脓毒症小鼠肺部炎症反应的抑制作用 ：张萍, 邹向前, 石庚生, 孙炳伟 【摘要】 目的： 研究外源性一氧化碳释放分子(carbon monoxidereleasing molecules2, CORM2)干预对脓毒症小鼠肺部炎症反应的抑制作用。方法: 18只雄性昆明种小鼠随机分为假手术组、CLP模型组和CORM2干预组。制作标准盲肠结扎穿孔(cecal ligation and puncture,CLP)模型,用CORM2进行干预,观察干预前后小鼠肺脏炎症反应的变化情况。分别于CLP术后6 h,12 h,24 h收集血浆、取肺组织,酶联免疫吸附(ELISA)法检测血浆中肿瘤坏死因子α(TNFα)和白细胞介素1β(IL1β)炎症因子的表达;检测肺组织中髓过氧化物酶(MPO)活性,观察中性粒细胞的聚集程度;测定肺组织湿干重比(1)、肺表面活性蛋白A(SPA)的表达。结果: 与假手术组比较,CLP模型组主要表现为肺组织微血管通透性增加,肺泡壁增厚,间质水肿,白细胞浸润,MPO活性明显增强;炎症因子TNFα,IL1β和IL6的表达水平升高(Plt;0.05);与CLP模型组比较,CORM2干预组肺间质水肿程度减轻,白细胞浸润明显减少,以上炎症因子的表达明显受到抑制(Plt;0.05)。结论: CDRM2可显著抑制脓毒症机体炎症细胞因子和ICAM1、SPA的表达,降低MPO活性,减少中性粒细胞的聚集,减轻组织炎症反应,对脓毒症小鼠肺部炎症反应有显著的抑制作用。 【关键词】 脓毒症; 肺; 一氧化碳; 炎症反应 　　[Abstract] Objective: To investigate the antiinflammatory effects of carbon monoxidereleasing molecules2(CORM2) to lung inflammation in sepsis mice. Methods: Eighteen male mice ly divided into 3 groups:sham group,CLP group and CORM2 group.To make standard cecal ligation and puncture models, exogenous carbon monoxide mation among those groups. After 6 h,12 h and 24 h of CLP operation, the expression of cytokines TNFα and IL1β in plasma samples atoxylineosin staining method and their myeloperoxidase activity, neutrophils infiltration in lung tissues detected. Results: pared group, the permeability of lung tissues in CLP group increased, the alveolar a and neutrophils infiltrated increasingly; the myeloperoxidase activity and the expression levels of inflammatory factors enhanced obviously.Contrarily, pared eability of lung tissues in CORM2 group decreased, the alveolar a and neutrophils infiltration yeloperoxidase activity and the expression levels of inflammatory factors decreased apparently. Conclusion: CORM2 had significant inhibitory effects to lung inflammation in sepsis mice by alleviating the expression levels of inflammatory factors and ICAM1, SPA, and by decreasing the leukocyte sequestration. 　　[Key onoxide; inflammation 　　脓毒症是由感染引起的系统性炎症反应综合征(system inflam