PTP1B基因多态性与儿童单纯性肥胖的关系.pdfVIP

中南大学学报 (医学版) JCentSouthUniv(MedSci) 9l5 AssociationofPTPlB genepolymorphism with obesityinChinesechildren M0Juan，WUJing，SUNZhixiang，YANGHaobo，LEIMinxiang，LIU Weifang (DepartmentofEndocrinology，XiangyaHospital，CentralSouthUniversity，Changsha410008，China) Abstract： Objective Toinvestigatethedistributioncharacteristicsofproteintyrosinephospha— tase1B (P7P』曰)geneIVS6+G82AandPro303PropolymorphismsinChinesechildrenanddetermine theeffectofpTPIB geneIVS6+G82A andPro303Propolymorphismsonthepathogenesisofchildhood obesity．M ethods A totalof147Chineseobeseand 118healthychildrenwererandomlyselectedand enrolledtoidentifyIVS6+G82A andPro303Progenotypesbypolymerasechainreaction—restrictionflag— mentlengthpolymorphism (PCR—RFLP)assay．Waistcircumference(WC)，waisttohipratio(WHR)， percentageofbodyfat(％BF)，systolicbloodpressure(SBP)，diastolicbloodpressure(DBP)，fasting plasmaglucose(FPG)，serumtriglycerides(TG)，totalcholesterol(TC)，highdensitylipoprotein—cho— lesterol(HDL—C)，lowdensitylipoprotein—cholesterol(LDL·C)，plasmafastinginsulin(FINS)，homeo— stasismodelassessmentforinsulinresistance(HOMA—IR)，andplasmaleptinwereexamined．Results TheallelefrequenciesofIVS6+G82A andPro303Prowere59．5％ and 19．4％ inobesechildren．and 53．4％ and 11．0％ in healthychildren．respectively．Thereweresignificantdifferencesinallelefre． quenciesofPro303Propolymorphism betweentheobeseandthecontrolgroup．Pro303Propolymorphism wasassociatedwithbodymassindex，WC，TG，andLDL—Cintheobesesubjects．Therewasnotdi— ffereneein thegenotypedistributionsorallelefrequenciesofIVS6 +G82A polymorphism between the obeseandthecontrolgroup．Furtheranalysisshowed noassociation between thegenotypesofIVS6 + G82Aandclinicalcharacteristicsintheobesesubjects．Thelinkagedi