补肾壮骨胶囊治疗KOA疗效观察及对MMP-1、TIMP-1、ADAMTS-4表达影响.pdf

Clinical Observation of Bushenzhuanggu Capsule in the Treatment of KOA and the Effect on the Expression of MMP-1, TIMP-1, ADAMTS-4 Speciality:Orthopaedics of TCM Author:Zhang xue xiang Tutor Prof:Zhang shi hua ABSTRACT Objective :To observe the efficacy of Bushenzhuanggu capsule(BSZGC) for treatment of knee osteoarthritis(KOA) and its mechanism.To observe effects on knee osteoarthritis chondrocytes ADAMTS-4, MMP-1, TIMP-1 expression. Methods: In the experiment study ,40 New Zealand rabbits were randomly divided into 4 groups:Group A (the normal group)，Group B (the model group)，Group C(the treated group) and Group D(the control group).KOA models were created in Group B, C and D by Hulth ’s operation methods. The rabbits in Group D were treated with Zhuangguguanjie pill (ZGGJP) and BSZGC was used in Group C. 8 weeks later, to make CR of the knee， Then to kill the rabbits and get the femoral condyle and measured MMP-1, TIMP-1, ADAMTS-4 positive cells. Clinical research applications BSZGC,ZGGJP treatment of KOA of the 30 patients in the control and the overall clinical efficacy observed symptoms and signs improve the situation. Results : The experiment study showed the model group significantly articular cartilage degeneration, HE staining and light microscopy MMP-1, TIMP-1, and ADAMTS-4 expression showed that the treatment group and model group were significantly different(P 0.01), treatment group and control group of related indicators no significant differences detected (P 0.05), Clinical studies have shown that application of BSZGC of KOA total efficiency and the control group were significan