实验室帮助 生物医学类（Laboratory assistance biomedical）.doc

实验室帮助 生物医学类（Laboratory assistance biomedical） Lab talent - Lilac Garden, oh dear 1, the extraction of plasmid DNA plasmid, the final step of alcohol volatilization is critical, basically is the decisive factor of its subsequent enzymatic reaction. So, this step, as far as possible volatile, a little longer, it is best to air conditioning blowing hot air, or 37 degrees warm box for a little longer time, I tried room temperature overnight, enzyme cut very well. Increase the AMP concentration to 200ug/ml, and get vaccinated before work... Do you want to be a smart lab lazy guy? 2013, 03, 20, 15:21:15 1. Plasmid DNA extraction When the plasmid is extracted, the final step of alcohol volatilization is critical, and is essentially the determinant of its subsequent enzymatic response. So, this step, as far as possible volatile, a little longer, it is best to air conditioning blowing hot air, or 37 degrees warm box for a little longer time, I tried room temperature overnight, enzyme cut very well. The concentration of AMP was increased to 200ug/ml, the inoculation before work, and the harvest of bacteria early in the morning. At the same time, although the OD was not high, the plasmid abundance was not normal. The volume of bacteria is small, and the volume of operation (Guan Shu) is small (less). A plasmid, if using chloroform and chloroform extraction atmosphere two step, and then look at the way, in strict accordance with the reference book above the operation, the plasmid than any plasmid kit, I feel a little better, I used a kit, compared after good as my laptop later, never used. 2, Western Blot When Western blot, people tend to develop a resistance, anti two concentration, closing time, exposure time and so on, and a condition which requires each transformation from the new running glue, transfer membrane, even to extract the protein, it will waste a lot of time. In fact, there is absolutely no need for that. Once the film is transferred, the PVDF film is dried, cut