骨缺损修复过程中相关生长因子基因表达.docVIP

骨缺损修复过程中相关生长因子基因表达摘要：目的：通过活血中药（骨碎补、续断、自然铜、 土鳖虫)对普通家兔骨缺损模型的喂养，探讨PDGF和IGF-I基因对骨缺损修复的影响。方法：健康家兔36只，其中24只按3天、1、2、4、6、8周分为6组，每组4只中再分实验组和对照组，每组2只；另外12只健康家兔为松质骨提供组，实验组和对照组普通家兔均按常规手术要求对右侧桡骨中段制成15mm的骨缺损，松质骨提供组普通家兔处死后取髂骨制成颗粒状骨植入上述骨缺损处，实验组用活血中药配置复合饲料喂养，对照组用普通饲料喂养，于术后3天、1、2、4、6、8周处死取骨缺损处移植骨痂标本做PDGF和IGF-I基因的动态定量PCR。结果：对PDGF和IGF-I的目的基因，校正结果后，以正常骨组织拷贝数为基础，其他各组与其比值：对照组3天、1、2、4、6、8周PDGF基因分别为1.00、1.70； 2.54； 1.72； 3.63；0.97;实验组为1.01、1.95、5.98、11.79、14.96、3.75. IGF-I对照组基因分别是1.00、3.03； 4.96； 1.14； 2.67；0.60；0.12；实验组为0.12、2.04、4、43、10.00、19.83、3.39。.结论：活血中药（骨碎补、续断、自然铜、 土鳖虫)能够明显增加PDGF和IGF-I的分泌量，并提高骨组织修复质量和对成骨有显著的影响。 关键词：血小板衍生生长因子；胰岛素样生长因子；定量聚合酶链反应 Abstract: Objective: To investigate the effect of platelet derived growth factor and insulin growth factor-I duringthe feeding model ofbonedefect with Chinese drug （gusuibu ;xuduan; nature copper; ground beetle ）Methods：Thirty-six health family rabbites, twenty-four of them family rabbites were randomly divided into six groups by three days 、1st、2nd、4th、6th、8th week, every group is four ,four family rabbites were randomly divided into test group and control group,every group is two , the other twelve family rabbites provide homologous spongy bone. family rabbites of test and control were made 15mm bone defect in their middle radius by common operation，The twelve family rabbites were killed offer homologous ilium spongy to implant obove bone defect, The control team was fed with common feed and the test team was fed with Chiense drug mixture feed. After they were killed in the three days 、1st、2nd、4th、6th、8th week and observe platelet derived growth factor and insulin growth factor-I of test group and control group by platelet derived growth factor and insulin growth factor-I dynamic quantification analysis of bone specimen. Results: After proofread of platelet derived growth factor and insulin growth factor-I , the ratio is test groups copiescomparing with normal bone copies, PDGF is 1.00、1.70； 2.54； 1.72； 3.63；0.97in control group and 1.0