儿童感染肺炎支原体后细胞免疫状态的变化及临床意义.pdfVIP

L 5 · 689· 儿童感染肺炎支原体后细胞免疫状态的变化及临床意义 林茹珠 邓向红 何洁冰① 蔡仕俊 曹建伟 毛静媛 广东省中山市人民医院儿科 528403 中国图书分类号 R725．6 文献标识码 B 文章编号 101114,111(2008)05-0689-03 【摘 要】 目的：探讨儿童感染支原体肺炎后细胞免疫功能的变化及临床意义。方法：分别采用流式细胞术和细胞酶免 疫分析法，对38例肺炎支原体感染患儿的T淋巴细胞亚群 (CD 3+、CD 4+、CD 8+CD 28+、CD 8+CD 28一)的表达和红细 胞补体受体1(CR 1，即CD 35)进行分子定量检测，45例普通肺炎患儿作为对照组检测上述指标。结果：支原体肺炎组患儿急 性期红细胞CR1表达明显下降，CD 3+、CD 4+、CD 8+CD 28+表达减少，而CD 8+CD 28一表达升高，与对照组比较有显著 性差异 (P0．01)。在恢复期上述情况有所改善，但与对照组相比仍有显著性差异 (P0．05)，相关分析表明红细胞CR 1与 CD 8+CD28一呈负相关 (R=一0．512，P0．05)。结论：支原体肺炎儿童存在红细胞免疫功能低下及T淋巴细胞活化异常，其 中CD 8+CD 28一细胞群的活化障碍和红细胞免疫功能的持续异常，可能是发生继发性过敏性咳嗽的重要细胞免疫学基础。 【关键词】 肺炎支原体 红细胞补体受体 淋巴细胞亚群 Changes and clinical significance of cytological immune function in children infected with mycoplasma pneumoniae LIN Ru—Zhu，DENG Xiang—Hong，HE Jie—Bing．Pediatrics Department ofZhongshan City People~Hospital，Guan— gzhou 528405，Guangdong，China (Abstract] Objective：To study the changes and clinical significance of cytological immune function in children suffered from my- coplasma pneumonia(MP)．Methods：38 cases of MP and 45 cases of common pneumonia were included．T lymphocytes subsets，inclu— ding CD3+，CD4+，CD8+CD28+、CD8+CD28一expression on the peripheral blood mononuclear cells were determined with flow cy· tomctry．The number of erythrocyte CR1(ECR1)was quantitatively assayed through alkaline phosphatasc conjugated to the first and second antibodies．Results：The expression of ECR1 and CD3+，CD4+，CD8+CD28+in MP individuals was obviously lower than those in control group during acute phase，while that of CD8+CD28一was higher in MP group than those in control group(P0．O1)．The difference was decreased during the recovering l~hasc with Stastical significance(P0．05)．Relative analysis showed there Was negative relativity between ECR1 and CD8+CD28一．Conclusion：There is disturbance ofimmune function in children llI MP，which focus on the