电离辐射对小鼠肠道隐窝素4表达的影响及意义-第三军医大学学报.DOCVIP

DOI：10.16016/j.1000-5404.201410080 电离辐射对小鼠肠道隐窝素4表达的影响及意义 曹 乐，王 成，申明强，王艾平，陈 芳，耿 放，陈 默，张乃心，粟永萍，程天民，王军平 （400038重庆，第三军医大学0.01）；辐照小鼠血清LPS含量在9 h后显著增加（P 0.05），72 h后升高最为明显（P 0.01）；Crp4对条件致病菌（大肠杆菌、粪肠球菌）的抗菌活性显著强于益生菌（乳酸杆菌、双歧杆菌），并能抑制LPS诱导单核巨噬细胞IL-1β的生成（P 0.01）。结论 电离辐射可以诱导小鼠肠道隐窝素Crp4的表达上调，Crp4表达上调可发挥调节肠道菌群、减轻机体炎症反应的作用。 [关键词] 电离辐射；隐窝素4；抗菌；LPS；IL-1β [中图法分类号] [文献标志码] A Alteration and Significance of small intestinal cryptdin 4 in ionizing-radiated mice Cao Le, Wang Cheng, Shen Mingqiang, Wang Aiping, Chen Fang, Geng Fang, Chen Mo, Zhang Naixin, Su Yongping, Cheng Tianmin, Wang Junping (State Key Laboratory of Trauma, Burns and Combined Injury, College of Preventive Medicine, Institute of Combined Injury, Third Military Medical University; Chongqing Engineering Research Center for Nanomedicine, Chongqing, 40038, China) [Abstract] Objective To observe the effect of ionizing radiation on the expression of mouse cryptdin 4 (Crp4) and explore the biological significance. Methods BALB/c mice (8 weeks-old) were randomly divided into a control group (n=5) and a radiated group (n=15), and the radiated group was treated by total-body ionizing radiation (8Gy X-ray). The small intestinal tissue and eyeball blood were collected before and after ionizing radiation (at 9 h, 24 h, and 72 h). Immunohistochemistry and Western blot were employed to detect the alteration of Crp4 in mouse small intestine. Quantitative chromogenic tachypleus amebocyte lysate was used to analyze the alteration of lipopolysaccharide (LPS) in blood serum. Crp4 peptide was obtained through chemical synthesis. Colony counting method was employed to assess the antibacterial activity of Crp4 against Escherichia coli, Enterococcus faecalis, Lactobacillus acidophilusm, and Bifidobacterium bifidum. Also, the effect of Crp4 on the release of IL-1β from mouse RAW264.7 cells that were simulated with LPS was evaluated. Results The expression of Crp4 in the mouse small intestine was increased after ion