肝脏X受体β对围生期小鼠海马细胞增殖以及放射状胶质细胞纤维形成的影响.docVIP

肝脏X受体β对围生期小鼠海马细胞增殖以及放射状胶质细胞纤维形成的影响 郭 亮1，黄 伟1，徐 培2，范晓棠2，徐海伟3*(400038 重庆，第三军医大学：基础医学部生理学教研室1，基础医学部组织胚胎学教研室2，西南医院眼科3) [摘要] 目的 观察肝脏X受体β（liver X receptor (， LXR(）Objective This study aimed to determine the effect of LXRβ on the cell proliferation and formation of radial glial fibers in the dentate gyrus ( DG ) of perinatal hippocampus . Methods The markers for cell proliferation (PCNA) and radial glial cells such as BLBP and Nestin were detected in the DG of LXRβ knockout mice and WT controls at Embryonic(E) 18.5 and Postnatal(P2) with immunohistochemistry. Results LXRβ was expressed in the hippocampus both at E18.5 and P2 with high level, of which densely stained positive cells mainly localized in the dentate gyrus (DG). At E18.5, loss of LXRβ induced the decreased number in the PCNA positive neurons(P0.01) and radial glial fibers labeled by BLBP (P0.05) compared to WT littermates, however ,it didn’t affect the number of radial glial cell bodies(P0.05). At P2, compared to E18.5, the number of PCNA positive neurons in the DG was slightly increased, which was decreased by loss of LXRβ(P0.05). Moreover, the morphology of BLBP labeled radial glia cells in the DG at P2 was different from that in the DG at E18.5 , of which bodies were slightly increased, and radial glial fibers disrupted. Loss of LXRβ induced the significant decrease in the number of radial glial fibers(P0.05), although did not affect radial glial cell bodies in the DG(P0.05). As another marker for labeling radial glial cells, Nestin has been shown to express in the similar expression pattern to that of BLBP, the radial glial fibers labeled by nestin antibody were disrupted in the LXRβ knockout mice both at E18.5d and P2. Conclusion LXRβ plays important role in the cell proliferation and formation of the fibers of radial glial cells in the DG of perinatal hippocampus. Key Words: Liver X Receptor β（LXRβ）, Radial glial cells, hippocampus, development Supported by National Basic Research Program of China (97