慢性焦虑应激大鼠行为学变化及高架十字迷宫测评.docVIP

慢性焦虑应激大鼠行为学变化及高架十字迷宫测评(1.北京中医药大学,北京100029;2.北京中医药大学第三附属医院,北京100029) 摘 要:目的:以慢性焦虑应激大鼠为载体,探讨其焦虑状态及行为学的变化,旨在探索能较好模拟广泛性焦虑障碍的动物模型。方法:采用慢性情绪应激的方法建立焦虑大鼠模型,以国际公认的高架十字迷宫试验对其行为学进行评价。结果:应激后大鼠处于易激惹的状态,表现出频繁的修饰和攻击行为,经高架十字迷宫测试发现大鼠较少进入并停留于开放臂中,倾向于探究封闭臂,且在封闭臂的停留时间延长。结论:不确定性空瓶刺激作为一种慢性心理应激模型,从病因学上较好模拟了广泛性焦虑障碍的发病状态和行为学表现。 关键词:焦虑模型;行为学;慢性情绪应激;高架十字迷宫? 中图分类号:R332 文献标识码:A 文章编号:1673-7717(2010)04-0711-03 The Changes in Behaviour of Rats with Chronic Stress-induced Anxiety and Behavioural Evaluation of The Elevated Plus-maze Test System LI Ning 1,TANG Qi?sheng2,ZHAO Rui?zhen2 , GOU Sheng?le1 (1. Beijing University of Chinese Medicine, Beijing 100029,China; 2. The Third Hospital Affiliated to Beijing University of Chinese Medicine, Beijing 100029,China) Abstract:Objective:To study the changes in behaviour of rats model with chronic stress-induced anxiety and to explore a appropriate animal model for GAD. Methods:Rats model was established by using chronic emotional stress-induced anxiety method, then researchers observed behavioural changes of model rats and evaluation of elevated plus-maze test system. Results:Rats resceiving emotional stress was in a state of great agitation,who manifested obvious grooming and attacking behaviour.Fewer rats entered and remained in the open arm,and tended to explore and stay in the closed arm.Conclusion:Unpredictable bottle stimulation as a methd of chronic emotional stress better simulated the incidence and manifestation of generalized anxiety disorder from etiology. ? Key words:anxiety model; behaviour;chronic emotional stress; elevated plus?maze 收稿日期:2009-11-21 基金项目:国家自然科学基金资助项目 作者简介:李宁(1980-),女,河南许昌人,博士研究生,研究方向:中医药防治脑血管疾病。 通讯作者:唐启盛(1956-),男,黑龙江人,教授,博士研究生导师,研究方向:中医药防治脑血管。 广泛性焦虑障碍(generalized anxiety disorder,GAD)是以过度和持续性焦虑或反复发作的惊恐不安为主要特征的神经症,常伴有植物神经系统症状和运动性紧张。随着临床和基础实验研究的不断深入与完善,人们对于本病也有了较为系统而全面的认识。现代医学研究表明,其发病涉及生物、心理、社会等诸多方面的因素,但确切的病因和发病机制至今尚未彻底阐明。焦虑动物模型作为基础实验研究的平台与载体,在探索焦虑障碍的病因、发病机制以及新药研发等方面发挥着重要作用。