白腐真菌菌株共培养降解玉米秸秆的研究.pdfVIP

安徽农业科学，Journal of?mhai A ．Sd．2008，36(4)：1327—1329 责任编辑 陈玉敏 责任校对 马君叶 白腐真菌菌株共培养降解玉米秸秆的研究 刘坤，李会宣，李敬(河北经贸大学生物科学与工程学院，河北石家庄050061) 摘要 [目的]探究白腐真菌菌株共培养对降解玉米秸秆的影响。[方法]以白腐真菌(F6和，，9)为供试菌株，采用分光光度法、DNS法、 凯民定氯法、索民提取法等，分别测定两菌共同降解玉米秸秆时纤维素酶和木质素酶的酶活力、全纤维素的降解率、粗蛋白和粗脂肪的 增加率，并与两菌分别培养进行综合比较。[结果]两菌共培养的最佳条件为pH值5．5、含水量60％、菌量(L-9／P-6)l：3、合氮量2．5％；共 培养后粗蛋白含量和粗纤维含量分别提高了35、6％和3．6％，粗纤维含量降低了21．3％。[结论]从总体思路考虑，两茵共培养优于单独 培养：该试验同时获得了1种高蛋白、多营养的饲料栽体，为研究玉米秸秆饲料奠定了基础。 关键词 白腐真菌；共培养；玉米秸秆 中图分类号 S182 文献标识码 A 文章编号 0517—6611(2O08)04—01327—03 Studies ontheCo-culture ofWhiteRot F瑚晒forD瞪rad Corn Stalk 【J[U Kun et al (College ofBiological Science and Engineering，Hebei University ofEconomies and Trade，Shijiazhuarrg，f{ebei 05OO61) Abstract lObjectivej The aim ofthe research was to explore the effects of the co-culture of white rot fungi on degrading coin stalk、lMethodj With white rot血1gi(P一6 and ．9)as tested strains，spectmphotometry，DNS n 山od，kjeldahlⅡ thed mid soxlrlet extraction n thed，etc．were used to deter— mine the activities of cellIIIose and ligninase，the degradation rate ofholecallulos and the increasing rate of erode protein(CP)and crude fat(CF)respec— tively in co-culture of2fungifor degrading com stalk．、Andtheseindexeswere comprehensively comparedwiththoseinindividualfungi culhire．1Resultj n optimmn conditionsfor co-culture of2f~ngiwereasfollows：pH value of5．5．water content of60％，the dosage ratio of L一9toP一6 ofl：3 and nitro． gen content of 2．5％ ．After co-culture，CP content arid CF content were increased by 35．6％ and 3．6％ respectively，the content of crude fiber was de． creased by 21．3％．{Conclusion}Consideringfrom the general fllhrking，file co-culture of2 was betier than individtml culture．In the test，a kind of feed vector with hi出一protein and poly．nutrient was obtained，which laid the fotmdation for studying coin stalk feed。 Key words White rot fungi；( cultured：Com smlk 农作物秸秆是一种非常规性和非竞争性的饲料资源。 l。2。4 正交试验_4。影响纤维素酶