干旱胁迫对宁夏枸杞幼苗膜脂过氧化及抗氧化保护酶活性的影响.pdfVIP

安徽农业科学，Journal of~ahui Agn．Sci．2008，36(4)：1343一l344，1552 责任编辑 刘永 责任校对 马君叶 干旱胁迫对宁夏枸杞幼苗膜脂过氧化及抗氧化保护酶活性的影响 郑国琦，谢亚军(宁夏大学生命科学学院，宁夏银J『1 750o21) 摘要 [目的]探究干旱胁迫对宁夏枸杞( 6nr如nM L．)幼苗抗氧化保护酶活性的影响，为枸杞的节水灌溉提供理论依据。『方法] 利用不同程度的干旱胁迫处理盆栽枸杞幼苗，分别在处理后第1…3 6 12 d测定枸杞幼苗叶片的膜透性、可溶性蛋白含量、超氧化物歧化 酶S0D活性、放氧速率及膜质过氧化产物MDA含量。[结果]宁夏枸杞具有较强的抗旱性，在干旱胁迫程度小于田间最大持水量45％的 条件下，宁夏枸杞以上各项指标与对照相比差异不显著，而在干旱胁迫程度大于田间最大持水量45％的条件下，各项指标变化显著。 [结论]田间最大持水量45％是宁夏构杞正常生长的临界值。当干旱胁迫程度低于该值时，宁夏枸杞能够正常生长，而当提高干旱胁迫 程度时，宁夏枸杞生长受到一定程度的影响。 关键词 干旱胁迫：宁夏枸杞；膜脂过氧化；抗氧化酶 中图分类号 Q945；Q946 文献标识码 A 文章编号 0517—6611(2o08)o4—01343—02 Effects ofDrought Stress on the 0翰I驴 ofMembrane-lipid Peroxidation and Antioxidase Activity in the Seedlings of Lycium barbarum L． 刁l皿 JG Guo-qi et al (Life Science School of Ningxia University．Yinchuan，Nin~a 750021) Abstract lObjectivej 111e research ain~xtto explorethe effects ofarought stress onthe activity ofthe antioxidation protective enzy／l~inthe secdl~cs of Lyci~ barbamm L．and provide theoretical basis f0r water-saving irrigation of L．barbarttm．I Methodl e potted seccU~gs of L．barbarum were treated with different degrees of drought stress，and the meITlbrane perrreability，the content of soluble protein，the activity of supemxide dismutase (S0D)，the oxygen evolution rate and the content ofmembrane—lipid supemxide MDA were detennined on the lst，3rd，6th and l2th d after trca~ents． 【Resultj L．borbarum had strongerdrought-resistance．whenthe degree of dro~t stresswaslowerthan45％ofthemaximumwater-holai~g capacityin the field．the above indexes in L．barbarum were not significantly different from that ofCK．When the degree ofdrought stress was higher than45％ of the maximum water-holding capacity in the field，all the indexes were significandy ch~ed，【Conclusion]45％0f llle maximum water-holding capacity in the field Was the critical value for the normal growth of L．barbarum，When the degree ofdrought stress Was lower than this value．L，barbaru