海藻酸钠-多聚赖氨酸-海藻酸钠微囊化大鼠卵巢激素分泌细胞异体移植对垂体形态学的影响.pdfVIP

中国组织工程研究与临床康复第12 举第I 蔚 2008-01-01 出版 @定再复基础实验 Joumal 01 Clinical Rehabilitative 7lssue Engineering R田earch Januaη叶， 2008 协1.12， NO.l 海藻酸纳-多聚赖氨酸-海藻酸纳微囊化大鼠卵巢激素分泌细胞异体移植对 垂体形态学的影响材 吴 桐，翁 静，刘 玲，史小林 Morphological changes in pituitary foUowing aUotransplant of alginate-polylysine-alginate microencapsulated ovarian ceUs in rats Wu Tong. Weng Jing. Liu Ling. Shi Xiao-lin Abstract cer阳ofRepn:也由四 AIM: It is confirmed 也at a1ginate-po1y1ysine-a1ginate (APA) microω.psu1e has good biωompatibi1ity and no obvious toxic side effect. M崎cine， 臼E曲创 APAmiαoencapsu1ati∞ ωn pro10ng the surviva1 time of allotransp1ants. This study conducted an allo国nsp1antation aft町 APA M目拉:aI Uni响:rstty， B回iji鸣 1000ω， micr民:ncapsu1ated rat ovarian cells 缸e cu1tured in vitro. exp10red 也e feasibi1ity of APA micr民组ω，psu1ated rat ovarianωlls in cbina tr臼恤ent of ovariectomized rats and studied 也e morpho10gy changes ofthe pituitary, Wu T，吨食，如句吨 METHODS: The experiment w挝C∞ducted in the Center of Reproductive Medici肘. Capital Mediω1 University 企om March 2006 to 量r ma脚s degree, Septemb町 2007①Experimental anirnals: 四irty female Wistar rats were di功ded randomly into 3 groups (1 0 rats each group): ∞ntrol C自由:r ofR句宽四luctive group. ovariectomy group and implantation group. Experimental procedures w町e in accordance wi曲曲ee由1ω1 stand缸d of anirnals Medicine, C咿创 ②M咄咄In ovariectomy group. ovaries on both sides were e刷刷刷at sterile. In implantation group. 也e P2 generation of rat M目拉:aI Univer岳飞y， ovarian cells w町e microenω，psulated by APA , and cultured in vitro. We collected 也e microenω，psulated cells culture medium at 24 闯闯 l侃仰， cbina ho田百 to test 出e secretion of es田diol (E2). And 出m 出e mlcroenω.psulated ovarian 臼lIs