组胺对分化的3T3L1细胞UCP2表达的影响.pdfVIP

中南药学2008年2月第6卷第1期 Central South Pharmacy．February 2008，Vo1．6 No．1 组胺对分化的3T3L1细胞UCP2表达的影响 曾慧，杨智，黄琼，尹继业，周宏灏，刘昭前 (中南大学I临床药理研究所，长沙 410078) 摘要：目的 探索组胺对脂肪细胞能量代谢的影响及其作用机制。方法 培养3T3L1细胞，诱导分化为白色脂肪 细胞，运用RT-PCR以及Western-blot技术检测组胺和苯海拉明对白色脂肪细胞中UCP2 mRNA水平和蛋白质表 达水平的影响。结果 白色脂肪细胞在不同剂量组组胺的刺激下，UCP2的表达值在不同剂量组之间存在显著差异 (PO．05)，UCP2的表达水平随着组胺剂量的增加而增加，在组胺剂量为30 ffmol·L 时达到最高值；在相同剂 量组胺下，观察不同时间对细胞内UCP2表达的影响，结果发现，不同时间组之间也存在显著性差异 (P0．05)， 当时间为6 h时，UCP2表达量达到最高；苯海拉明剂量依赖性地抑制脂肪细胞UCP2 mR NA水平和蛋白质的表 达。结论 组胺可以刺激分化成熟的3T3L1细胞中UCP2的表达，其机制与激活H 受体有关。 关键词：组胺；UCP2；分化；能量代谢；苯海拉明 中图分类号：R969 文献标识码：A 文章编号：1672—2981(2008)01—000l—O5 Effect of histamine on UCP2 expression in the differentiated 3T3L1 cells ZENG Hui，YANG Zhi，HUANG Qiong，YIN Ji ye，ZHOU Hong—hao，LIU Zhac~qian (Institute of Clinical Pharmacology，Central South University，Changsha 4 10078) Abstract：Objective To investigate the mechanism of energy metabolism induced by histamine in the differentiated 3T3L1 cells．Methods We induced 3T3I 1 cells as preadipocytes into differentiated adipocytes．The total RNA from the differentia— ted 3T3I 1 cells was extracted and semiquantitative R，r_PCR was performed to deternqine the level of UCP2 mRN 1、he ex— pression level of 2 protein was deternqined by western blot．Results Histamine at 30 ffmol·L～ significantly in— creased the expression level of UCP2 mRNA and UCP2 protein and their expression level reached a peak value．There were significant differences in the expression level of UCP2 mRNA and UCP2 protein in the adipocytes treated with 30 ffmol·I histamine at various time points within 48 h，respectively，and their expression level reached a peak value at 6 h incubation．Diphenhydramine(an Hl receptor antagonist)significantly decreased the expression levels of UCP2 mRNA and