糖尿病肾病肾组织TRAIL表达与肾小管间质损伤的关系.docVIP

DOI：10.16016/j.1000-5404.201408168 糖尿病肾病肾组织TRAIL表达与肾小管间质损伤的关系 陈 佳，戴欢子，霍本刚，杨聚荣，林利容，张建国，郑卢权，何娅妮 （400042 重庆，第三军医大学大坪医院野战外科研究所肾内科） [摘要] 目的 探讨肿瘤坏死因子相关的凋亡诱导配体（tumor necrosis factor related apoptosis inducing ligand，TRAIL）在糖尿病肾病（diabetic nephropathy，DN）患者肾组织的表达以及与肾组织损伤之间的关系。方法 选取2010-2013年本科36例患有2型糖尿病经肾活检诊断为DN患者，9例肾错构瘤或肾癌切除术患者经病理检查为正常组织作为对照。收集各组患者人口学资料、临床数据，免疫组织化学检测肾组织TRAIL表达，并与临床数据及肾组织病理损伤进行相关性分析。结果 DN患者TRAIL主要表达于肾小管上皮细胞细胞质和/或细胞膜，其表达水平随着肾小管间质损伤的加重而逐渐增加；与收缩压、尿蛋白定量、尿NAG、尿蛋白/肌酐、尿酸、尿素氮、血肌酐、光抑素C呈正相关，与eGFR呈负相关（r=-0.936，P0.01）。结论 DN肾组织TRAIL表达与肾组织损伤程度及肾功能密切相关，提示TRAIL在DN疾病进展中具有重要作用。 [关键词] 糖尿病肾病；肿瘤坏死因子相关的凋亡诱导配体；肾小管间质损伤 [中图法分类号] R365；R587.2；R692.6 [文献标志码] A Relationship between TRAIL expression and renal tublointerstitial injury in diabetic nephropathy Chen Jia, Dai Huanzi, Huo Bengang, Yang Jurong, Lin Lirong, Zhang Jianguo, Zheng Luquan, He Yani (Department of Nephrology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, China) [Abstract] Objective To investigate the expression of tumor necrosis factor related apoptosis inducing ligand (TRAIL) in patients with diabetic nephropathy (DN) and the relationship between TRAIL expression and renal tissue injury. Methods Thirty-six patients with type 2 DN were recruited for this study and nine patients with normal kidney tissues after renal hamartoma or cancer resection were collected as the control group. All the patients were selected from the department of nephrology in Daping Hospital from January, 2010 to December, 2013. The demographic data and clinical data of all the patients were collected. TRAIL expression in renal tissues was detected with immunohistochemical method, and its correlation with clinical data and renal tissue injury was analyzed. Results TRAIL was mainly expressed in cell membrane and/or cytoplasm of renal tubular epithelial cells, and its expression level rose along with the severity increase of renal tubulointerstitial injury. TRAIL expressi